Entering edit mode
3.1 years ago
LittLe
•
0
Hi everyone, I am using psiblast (Position-Specific Iterated BLAST 2.13.0+) to derive a PSSM file from multiple alignment file downloaded from the NCBI official website, but I always get this error:
BLAST query error: CAlnReader::GetSeqEntry(): Seq_entry is not available until after Read()
The code I used as below:
psiblast -in_msa sample_msa.txt -db nr -out out.txt
the sample_msa.txt is here:
26SPS9_Hs IHAAEEKDWKTAYSYFYEAFEGYdsidspkaitslkymllckimlntpedvqalvsgkla
F57B9_Ce LHAADEKDFKTAFSYFYEAFEGYdsvdekvsaltalkymllckvmldlpdevnsllsakl
YDL097c_Sc ILHCEDKDYKTAFSYFFESFESYhnltthnsyekacqvlkymllskimlnliddvkniln
YMJ5_Ce LYSAEERDYKTSFSYFYEAFEGFasigdkinatsalkymilckimlneteqlagllaake
Does anybody know the problem?
