Error in plot + xlab(xlab) : non-numeric argument to binary operator

Question

[imran]

My Volcano plot is working fine for the argument on one shape for all as below;

EnhancedVolcano(res_df,
lab = res_df$gene_sym,
x = 'log2FoldChange',
y = 'pvalue',
title = 'MSC Cellular RNA vs MSC Exosome',
pCutoff = 0.05,
FCcutoff = 1,
pointSize = 3.0,
labSize = 6.0,
shape = 23,
colAlpha = 1)

But when I pass the argument for different shapes for my data it gives me the error;

Error in plot + xlab(xlab) : non-numeric argument to binary operator.

Code is as below

EnhancedVolcano(res_df,
lab = res_df$gene_sym,
x = 'log2FoldChange',
y = 'pvalue',
pCutoff = 0.05,
FCcutoff = 1,
cutoffLineType = 'twodash',
cutoffLineWidth = 0.8,
pointSize = 4.0,
labSize = 6.0,
shape = c(23,8,29),
colAlpha = 1,
legendLabels=c('Not sig.','Log (base 2) FC','p-value',
'p-value & Log (base 2) FC'),
legendPosition = 'right',
legendLabSize = 16,
legendIconSize = 5.0)

head(res_df, n = 20)
 baseMean log2FoldChange    lfcSE        stat    pvalue      padj sig  gene_sym
ENST00000003583.12 126.0788    -0.21325126 2.410631 -0.08846284 0.9295088 0.9869099  no     STPG1
ENST00000003912.7  420.2135    -1.30895409 2.511233 -0.52123967 0.6021998 0.9859208  no    NIPAL3
ENST00000009105.5  231.4948    -1.75115300 2.472340 -0.70829769 0.4787604 0.9859208  no    CAMK1G
ENST00000011700.10 651.5186    -0.85162939 2.248348 -0.37878013 0.7048511 0.9859208  no    VPS13D
ENST00000040877.2  339.6129    -0.90238534 1.968157 -0.45849246 0.6465987 0.9859208  no    TARBP1
ENST00000054666.11 209.4070    -0.84781944 1.510004 -0.56146826 0.5744784 0.9859208  no     VAMP3
ENST00000072644.7  136.7335    -1.16720108 2.189308 -0.53313708 0.5939387 0.9859208  no     YIPF1
ENST00000078527.8  150.9054    -0.68232223 1.899988 -0.35911930 0.7195058 0.9859208  no      PIGV
ENST00000164247.5  413.2861    -1.45587717 2.516066 -0.57863225 0.5628373 0.9859208  no    KCNAB2
ENST00000166244.8  237.0218    -0.38783566 2.470313 -0.15699862 0.8752459 0.9859208  no     EPHA8
ENST00000167825.5  221.8213    -0.84683015 2.259946 -0.37471257 0.7078742 0.9859208  no ARHGEF10L
ENST00000196061.5  438.2576    -0.13550066 1.473975 -0.09192872 0.9267547 0.9866210  no     PLOD1
ENST00000207157.7  205.4427    -0.58784413 1.960836 -0.29979257 0.7643354 0.9859208  no     TBX15
ENST00000209884.5  292.1704    -1.49140772 2.008418 -0.74257847 0.4577369 0.9859208  no    KLHL20
ENST00000209929.10 246.3748    -0.38738951 2.473644 -0.15660679 0.8755548 0.9859208  no      FMO2
ENST00000212355.9  426.0076    -0.50231649 1.772641 -0.28337187 0.7768918 0.9859208  no    TGFBR3
ENST00000234590.10 444.9738     0.96619560 2.055609  0.47002896 0.6383343 0.9859208  no      ENO1
ENST00000234626.11 164.4949    -0.79541498 2.258505 -0.35218656 0.7246984 0.9859208  no      CDC7
ENST00000234668.6  131.0738    -0.21179566 2.142843 -0.09883861 0.9212664 0.9862636  no     SYDE2
ENST00000234677.7  146.4957    -0.02758746 1.475394 -0.01869836 0.9850817 0.9965418  no      SARS

Please tell me what I am doing wrong, there are three sets in data Nonsignificant (NS), Log2Fc. pvalue, I want different shapes for all these in my volcano plot. Also how can I remove label enhanced volcano from the plot

Thanks

[rpolicastro]

You have 4 categories in legendLabels so you need 4 shape values.