Does chromosome order matter when combining individual primary assembly files?
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23 months ago
iahuja • 0

I'm attempting to create a salmon index file for gallus gallus, for which I need both the genome and transcriptome files; from Ensembl, I was able to download the cdna.all transcriptome file as well as the individual chromosome primary assembly files (primary_assembly1–39, MT, W, and Z). To create one primary assembly genome file, does it matter in which order I concatenate the files? Or for this purpose, would just using the toplevel file be acceptable?

ensembl genome rna-seq • 490 views
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