miRNA annotation miRge3.0
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5 months ago
bas2020 ▴ 60

Hi everybody,

I aligned murine miRNA using miRge3.0. However, somehow the nomenclature/annotation seems to be odd. I could not find any rules for it online. Often I even couldnt find the miRNA in online databases. I want to use dbs like miRBase to search for known interactions with mRNA and to perform ontology analysis.

I will give you some examples:

Mmu-Mir-15-P1b_3p* -> what does the star stand for. I read that in former annotation it was used instead of 3p/5p. Since 3p is given it must have another meaning

Mmu-Mir-92-P1a_3p/92-P1b_3p -> I could not find 92-P1b, what does PXX mean at all? It is not the normal way to describe miRNA

Mmu-Mir-199-P1-v1_3p/199-P1-v2_3p/199-P1-v3_3p/199-P2-v1_3p/199-P2-v2_3p/199-P2-v3_3p/199-P3-v1_3p/199-P3-v2_3p/199-P3-v3_3p -> this is a terrible annotation, what does v stand for?

I guess, the annotation includes some nt or length variations. However, the rules are missing and I dont know how to break it down to a simple term, that is provided by the major online dbs and if necessary ignoring variations. I hope, someone can help me.

Best

Chris

miRge3 • 278 views
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Entering edit mode
5 months ago
Trivas ★ 1.7k

My guess is the P1a are referring to paralogs of the same miRNA, suggested by mirgenedb: https://mirgenedb.org/show/mmu/Mir-92-P1a. Probably poorly annotated so lots of revisions over time, hence the different versions.

IMO miRNA research is already nebulous, I would filter these out and focus on your other hits.

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