Entering edit mode
27 days ago
Herman Einstein
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0
I am studying gene A in WT and a gene B mutant strain. I did ChIP on gene A in WT and mutant followed by sequencing. For WT, I can capture the signal of gene A but a very low signal (similar to background) in the mutant. How do I know whether the low signal of gene B is not because of a failure of ChIP experiment, except by doing more replicates?
Have you tried using controls? A useful guide here.