Entering edit mode
13 months ago
Zhen
•
0
I want to see the splicing of my vector/reporter, after plasmid transfection and RNA extraction, I run the ONT direct RNA sequencing, I got the raw FASTQ file from Minion1B, and using minimap2 mapping to my reporter plasmid fasta file. After convert into sorted bam file, I loaded them into IGV, but I can not see the intron region.