umi tools RNA data [transcriptome alignment or genome alignment]
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8 months ago
Shiyi ▴ 40

I have ONT long reads RNA data with my own UMIs, I wonder if I should align to transcriptome or genome [minimap2]. My goal is primarily testing my UMI design and gene quantification, care a bit less about transcript quantification right now. Have anyone tried these and have a preference? Thank you.

ont umi_tools • 336 views
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