Normalisation for sample splice junction profiles
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Entering edit mode
7 months ago

Hi,

I want to create a matrix of junction splicing profiles of my samples

Rows as junctions Columns as samples

I will be using STAR sj.out.tab files as my input.

What should I use to normalise the JCs from each sample? Uniquely mapped reads? Total reads? Total splices?

Thanks

splicing normalisation RNAseq • 349 views
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