Hello all.
I’ve previously only worked with human genomic data, but I’m now working on a project that includes RNA-Seq data from mice. We’ve identified differential alternative splicing events in mice for a set of genes. In addition, we have RNA-Seq data from normal human samples collected at a comparable developmental time point and same tissue type.
Would it be informative to compare alternative splicing patterns between the human and wild-type mouse samples? I'm not very familiar with the mouse genome and am unsure how closely orthologous genes and their isoforms resemble those in humans.
Would it make more sense to focus on comparing annotated isoforms and gene expression levels, rather than attempting a direct comparison of alternative splicing events? The main idea is to provide some metric for similarity of alternative splicing between mice and human in our set of genes, although maybe this has already been done and there's no reason for additional analysis.
