How to convert demultiplexed Fastq to PRGs if Adaptor and Barcode information is not provided?
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3 months ago
s.pal2 • 0

I have downloaded a number of raw FASTQ files from SRA for UCES and intend to process them using PipeSnake to generate PRG (fasta) files. The associated paper mentions using custom, dual-indexed adapters and unique barcodes for each sample; however, this information is not available in the SRA metadata. I checked with the SRA team, and they mentioned that the samples have been demultiplexed, and so the adaptors have been removed. Is there a way to process these cleaned fastq files to convert them into PRGs for further analysis? Any suggestions in this regard will be highly appreciated. Thanks a lot,

Pipesnake Paleomix UCE Fastq • 2.2k views
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