Redox Ratio Analysis - RRApp for MatLab (V 1.3)

Question

[bm.nina]

I am trying to install the RRA app for redox ratiometric analysis. I am running MATLAB R2025a and using the most recent version provided in the repository (https://markfricker.org/77-2/software/redox-ratio-analysis/redox-ratio-analysis-software-download/). However, it appears that the package is no longer compatible with MATLAB, as I was able to install it. Still, it doesn't appear in the APPS tab. Can anyone recommend either a similar app or some troubleshooting options? Thank you in advance.

Answer 1

[Kevin Blighe]

Hi,

The RRA app from Mark Fricker's repository is quite outdated—last updated in 2017 for MATLAB R2017a. Given you're on R2025a, compatibility issues are likely the culprit, even if installation succeeds. MATLAB apps from older versions often fail to register properly in newer releases due to changes in the app framework.

First, troubleshoot by restarting MATLAB and checking the Apps gallery (click the arrow on the Apps tab). If it's still missing, run ver in the Command Window to confirm if the app/toolbox is listed. Also, ensure the installation path is added to MATLAB's path via addpath. If it's a .mlappinstall file, try reinstalling via the Add-On Explorer.

For alternatives, consider FIJI (ImageJ bundle), which supports ratiometric analysis via plugins like Ratio Plus or custom macros for redox ratios (e.g., NADH/FAD). It's free, actively maintained (latest v1.54 as of 2025), and handles bioimaging well without MATLAB dependency.

In MATLAB, you could script your own using Image Processing Toolbox: load images, compute ratios pixel-wise (e.g., ratio = imdivide(img1, img2)), and apply filters. This avoids obsolete apps.

If you share more error details, I can refine suggestions.

Kevin