Hy everyone, I am using IsoformSwitchAnalyzerR (ISAR) for my isoform switching study. One of the functions in this tool is that it takes Pfam output file and tells us which protein domains are present/absent in isoforms. I did Pfam analysis on my FASTA file on ubuntu and trying to load into results into ISAR for downstream analysis. but ISAR is showing parsing, file formatting issues and is unable to read it. I have downloaded the latest version of ISAR, ran Pfam twice but still the same issue. Raw Pfam file is in domtblout format and have 21 columns. ISAR accepts 16 column files. I have done that too. I am failing in doing analysis on the Pfam file. If anyone has ever worked with Pfam file or is familiar with ISAR, please reach out to help.
