Slam-Seq Conversion Rate per Read

Question

[Daniel]

Hello,

I've analyzed slam-seq data using the relatively older nf-core slamdunk pipeline. I've been told to proceed with the data because it's from a very high-impact publication, but I am confused on how the data passes QC. I thought that T>C conversion plots are very important to tell whether an slam-seq was successful, and to me, it seems that the time points here do not at all correlate with the conversion rates. Is there something I am missing? Thank you!

[michael.ante]

Try to switch to percentage view of this graph. Stacked bar charts with different heights can sometimes be misleading.