1 vs 1 DEG analysis in scrna seq data

Question

[carolofharvest]

Hello ,

I have only two sample and each from seperate condition. My library size in each sample is quite high. I want to make a DEG analysis between two of them and I know that we need at least 3 sample in each condition to be able to make a reliable comparison, But still I have to do it somehow. What can I do ? If i do random sampling from cells and create multiple pseudoreplicates from each sample I get too many DEGs,