NOVOPlasty assembly fails with “INVALID SEED” using first read as seed

Question

[Buddha]

Hello,

I am trying to assemble a complete mitochondrial genome using NOVOPlasty from filtered paired-end FASTQ files. The files I am using are:

ERR322446.filtered.A.fq

ERR322446.filtered.B.fq

seed file link: ERR322446_seed.fasta I used the first read sequence from ERR322446.filtered.A.fq as the seed in NOVOPlasty. However, the program returns the following error:

INVALID SEED, PLEASE TRY AGAIN WITH A NEW ONE

NovoPlasty Output log: log fle

I have checked that the seed sequence is in FASTA format with a proper header and matches the first read exactly. My NOVOPlasty configuration file is config.txt.

Access to all files: BioStar_ERR322446

I’m stuck and not sure what I’m missing. Has anyone encountered this issue or can suggest a reliable way to generate a valid seed for NOVOPlasty?

Thank you in advance!