interpretation of the results obtained with digital PCR

Question

[Lorenzo]

I'm using non-droplet digital PCR to identify the presence or absence of an RNA using the cDNA made from that RNA. I wanted to ask if the threshold can be set based on the internal negative control or the NTC. I need this because each sample has its own threshold, different from the others, and I can't interpret the data.

I'd also like to know if I can consider a sample positive with just one distribution, or if there must be a specific number of multiple distributions.

Thank you for your attention.