Homology Directed Repair in Crispr-Cas9 gene editing/deletion

Question

[hiname]

Can anyone give me clear-cut instructions on how I can design a homology arm for HDR after using Crispr-Cas9 to delete my target gene. How could i introduce the silent mutations in my arms, the flank sequences, everything. Should I clone my HDR template in a plasmid or not and how to do that?

[dthorbur]

You are asking for quite a lot of help here, and you haven't demonstrated any prior research. Whilst on SO, they do have a post describing how to ask a good question.

I would also say that this question is best for your PI, a postdoc, or a PhD student versed in CRISPR. Short of that, there are many resources from places like snapgene or benchling that show up with a simple google and would be a good starting point.