Entering edit mode
5 hours ago
graeme.thorn
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110
I'm designing an experiment to determine differentially methylated regions of DNA between two groups of tissues (lets call them A and B). Both A and B are from the same human organ (breast tissues), and will be Nanopore sequenced, so this is not array data, which the methylation literature seems to be focused on. Obviously, the difference is in the number of CpGs being assessed via sequencing rather than through a 450k array.
Given that we expect that there would be maybe 10-100 DMRs (possibly more, but the literature doesn't suggest this) between the two groups, how would we calculate how many samples in each group we would need to give us a specified detection power?
