User: Daniel

gravatar for Daniel
Daniel3.5k
Reputation:
3,470
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Trusted
Location:
Cardiff University
Last seen:
10 hours ago
Joined:
6 years, 11 months ago
Email:
d*****************@googlemail.com

Posts by Daniel

<prev • 358 results • page 1 of 36 • next >
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Comment: C: convert illumina to fastq Sanger
... Please explain what you are trying to achieve and why, so that we can help you, as this request is confusing. Do you have a bcl file? ...
written 7 weeks ago by Daniel3.5k
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Answer: A: FASTA file into excel file format?
... There are lots of jokes above, but the honest answer is that you do not want to do this. Once you have converted the file it would be so massive that it would impossible to open and use in excel before any other problems. Further, this is not an appropriate way to read the data, and 99.9% of the tim ...
written 3 months ago by Daniel3.5k
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Comment: C: Preparation for Bioinformatics.SE closing
... Seconding the 'finding my own posts' point, only yesterday I was trying to find an old answer of mine to put to a duplicate question and gave up after 5 mins of searching and scrolling because I couldn't find it. ...
written 6 months ago by Daniel3.5k
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Comment: C: Preparation for Bioinformatics.SE closing
... I think the difficulty searching adds to the repeated low quality questions. ...
written 6 months ago by Daniel3.5k
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Answer: A: Preparation for Bioinformatics.SE closing
... I think that could be solved quite easily with (1 ) a more restricted set of tags for questions to stop fragmenting, and (2) allowing them to be community-suggested so that new users don't have to be blamed for misattributing a question (this works quite well for games on steam) i.e. > **Based o ...
written 6 months ago by Daniel3.5k
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Comment: C: else condition in awk
... If it's not working don't click 'accept' and tell us what the error is. ...
written 8 months ago by Daniel3.5k
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Comment: C: How many lanes does the Nextseq (illumina) flow cell have?
... This is misleading, because although the NextSeq has 4 lanes, they aren't physically isolated and only one pooled library can be used, unlike the physical separation in the HiSeq. I think this is normally what people are looking for when asking about number of lanes. ...
written 9 months ago by Daniel3.5k
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Comment: C: What is the limiting factor for trimmomatic speed and how can it be increased?
... Fantastic, I'll give this a go! ...
written 9 months ago by Daniel3.5k
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What is the limiting factor for trimmomatic speed and how can it be increased?
... I'm using trimmomatic mainly to filter out adapters in the read through of my paired end illumina data. My command is as follows, and produces expected results: java -jar trimmomatic-0.33.jar PE 01_R1.fastq 01_R2.fastq 01_R1-trimpair.fastq 01_R1-trimunpair.fastq 01_R2-trimpair.fastq 01_R2-trim ...
trimmomatic fastq written 9 months ago by Daniel3.5k
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Answer: A: Progress bar to measure blast running time?
... I too am impatient and want to monitor how my runs are going. While what people are saying above is correct that there's not a built in method, what I normally do is use `watch` with either `grep -c ` or `wc -l` depending on your output format. But if you want to do it in your script, either read i ...
written 9 months ago by Daniel3.5k

Latest awards to Daniel

Commentator 6 months ago, created a comment with at least 3 up-votes. For C: Why Didn'T Biostar Join Stack Exchange?
Teacher 6 months ago, created an answer with at least 3 up-votes. For A: Convert Fas/Qual Into Fastq?
Appreciated 7 months ago, created a post with more than 5 votes. For A: Conferences On Bioinformatics
Scholar 9 months ago, created an answer that has been accepted. For A: Correcting sequence reads based on ORF disruption from reference sequence.
Student 11 months ago, asked a question with at least 3 up-votes. For Rdp, Greengenes And Arb-Silva. Are There Any Advantages Of One Over The Other?
Teacher 12 months ago, created an answer with at least 3 up-votes. For A: Convert Fas/Qual Into Fastq?
Good Question 14 months ago, asked a question that was upvoted at least 5 times. For Rdp, Greengenes And Arb-Silva. Are There Any Advantages Of One Over The Other?
Commentator 14 months ago, created a comment with at least 3 up-votes. For C: Why Didn'T Biostar Join Stack Exchange?
Popular Question 15 months ago, created a question with more than 1,000 views. For Error when fuzzy sequence matching using String::Approx in perl
Appreciated 16 months ago, created a post with more than 5 votes. For A: Conferences On Bioinformatics
Commentator 17 months ago, created a comment with at least 3 up-votes. For C: Why Didn'T Biostar Join Stack Exchange?
Teacher 19 months ago, created an answer with at least 3 up-votes. For A: Convert Fas/Qual Into Fastq?
Appreciated 19 months ago, created a post with more than 5 votes. For A: Conferences On Bioinformatics
Scholar 19 months ago, created an answer that has been accepted. For A: Is there a succinct method of isolating sequences phylogenetically similar to a
Scholar 19 months ago, created an answer that has been accepted. For A: Correcting sequence reads based on ORF disruption from reference sequence.

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