User: cvu

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cvu130
Reputation:
130
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India
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3 months, 3 weeks ago
Joined:
4 years, 10 months ago
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Posts by cvu

<prev • 65 results • page 1 of 7 • next >
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Comment: C: Removing columns coded by -,N or ? in Alignments
... You can use `sed` command if you just want to remove these characters `sed 's/[N|?|-]//g' filename` ...
written 5 months ago by cvu130
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Remove Sequences from existing Bowtie2 index
... Hello All, I've created a bowtie2 large index, Which is a huge data set (~100GB) it took approx 2days to generate a bowtie2 index. Now I came across problems with some sequences and I want to remove it from my existing index. Is there a way to remove sequences based on their identifiers directly ...
genome bowtie2 ngs index written 5 months ago by cvu130
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Answer: A: run gene enrichment analysis using metatranscriptomic data
... Did you resolve this problem? which OrgDb can be used? ...
written 13 months ago by cvu130
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bsseq fisherTest result
... Hi all, I've performed fisherTests() function of bsseq bioconductor package for two samples since I've only one replicate for each samples. but fishertest result gives only two columns p.value and log2OR and I want to know its corresponding chromosome and position information. also I want to filter ...
bsseq methylation bisulfite sequencing written 15 months ago by cvu130
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RRBS: import Bismark output to RnBeads
... Hello All, I am running Bisulfide sequencing samples. I've directional libraries with four single-ended fastq files for each sample and completed with bismark and got four bam files which i've merged and finally got one bam file for each sample. These are the commands I ran: bismark --genome_ ...
bsseq methylation bismark bisulfite sequencing written 15 months ago by cvu130 • updated 14 months ago by Biostar ♦♦ 20
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Comment: C: standalone BLAST parametes
... Thanks for the reply. I want only one best match for each protein, Hence I set hsp 1. ...
written 18 months ago by cvu130
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Comment: C: standalone BLAST parametes
... Thanks for the reply. I want perfect matches, but which parameters to set to get a good match? ...
written 18 months ago by cvu130
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standalone BLAST parametes
... Hi All, I've predicted genes in genome. Now I want to identify proteins, For that, I've blasted all predicted proteins against uniprot database. blast parameters blastp -query proteins.fasta -db Uniprotdb -max_target_seqs 1 -max_hsps 1 -out output.blastp -outfmt 6 -evalue 0.001 what should b ...
genome gene blast written 18 months ago by cvu130 • updated 18 months ago by Macspider2.8k
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Comment: C: which tool is used for separating forward and reverse from paired end fastq
... use SRA Toolkit https://www.ncbi.nlm.nih.gov/books/NBK158900/ and use fastq-dump, for example `fastq-dump -I --split-files SRR390728` to split paired end reads. ...
written 20 months ago by cvu130
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Comment: C: Predict Prion like Proteins (PLAAC)
... Thanks for the reply microfuge ...
written 20 months ago by cvu130

Latest awards to cvu

Scholar 9 months ago, created an answer that has been accepted. For A: how i can install bowtie?
Popular Question 13 months ago, created a question with more than 1,000 views. For how to map greengene ids to ncbi taxon ids ?
Great Question 15 months ago, created a question with more than 5,000 views. For Creating reference Index using Bowtie2
Epic Question 18 months ago, created a question with more than 10,000 views. For Bowtie2 parameters for best alignment
Popular Question 18 months ago, created a question with more than 1,000 views. For VariantRecalibrator Error message
Supporter 20 months ago, voted at least 25 times.
Great Question 21 months ago, created a question with more than 5,000 views. For Creating reference Index using Bowtie2
Popular Question 2.3 years ago, created a question with more than 1,000 views. For VariantRecalibrator Error message
Popular Question 2.3 years ago, created a question with more than 1,000 views. For Creating reference Index using Bowtie2
Popular Question 2.3 years ago, created a question with more than 1,000 views. For Samtools mpileup refrence fasta file input
Popular Question 2.4 years ago, created a question with more than 1,000 views. For Give hyperlink to pdf table perl
Popular Question 2.6 years ago, created a question with more than 1,000 views. For Comparing vcf with dbSNP
Great Question 3.0 years ago, created a question with more than 5,000 views. For Bowtie2 parameters for best alignment
Popular Question 3.3 years ago, created a question with more than 1,000 views. For Comparing vcf with dbSNP
Popular Question 3.3 years ago, created a question with more than 1,000 views. For Creating reference Index using Bowtie2
Popular Question 3.6 years ago, created a question with more than 1,000 views. For Bowtie2 parameters for best alignment
Popular Question 3.9 years ago, created a question with more than 1,000 views. For Creating reference Index using Bowtie2
Popular Question 4.0 years ago, created a question with more than 1,000 views. For Creating reference Index using Bowtie2
Popular Question 4.0 years ago, created a question with more than 1,000 views. For Bowtie2 parameters for best alignment

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