User: tiago211287
tiago211287 • 1.1k
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Posts by tiago211287
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3
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1
answer
111
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... Maybe Adobe Illustrator? ...
written 4 days ago by
tiago211287 • 1.1k
0
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0
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101
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... Maybe Helpful?
https://www.nature.com/articles/s41467-018-08023-x ...
written 4 days ago by
tiago211287 • 1.1k
0
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3
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1.6k
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3
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Comment:
C: ATAC-seq DE analysis
... Your link does not work anymore. Could you update it? Thanks! ...
written 3 months ago by
tiago211287 • 1.1k
0
votes
1
answer
331
views
1
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Comment:
C: bam file merge
... In http://www.htslib.org/doc/samtools-1.2.html,
it says,
> -h is specified the @SQ headers of input files will be merged into
> the specified header, otherwise they will be merged into a composite
> header created from the input headers. If in the process of merging
> @SQ lines for coor ...
written 7 months ago by
tiago211287 • 1.1k
0
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3
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736
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3
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Comment:
C: Filtering rRNA from RNAseq data
... You can normally align the reads against genome + annotation (gencode), using STAR for example which can count the number of reads/feature. Then just check the annotated rRNA gene count percentage. You would need "good" samples as a control of what is normal/bad. ...
written 8 months ago by
tiago211287 • 1.1k
0
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187
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... Have you already called your peaks? You can Use MACS2 for peak calling. To only then, choose strong peak signals. I would also use deeptools to access Chip-Seq Sequencing quality and statistics. ...
written 8 months ago by
tiago211287 • 1.1k
0
votes
3
answers
742
views
3
answers
... Actually, there is, using max-procs 1, you avoid loops. ...
written 12 months ago by
tiago211287 • 1.1k
1
vote
1
answer
596
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1
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... Are you doing synteny analysis? ...
written 12 months ago by
tiago211287 • 1.1k
0
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437
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0
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... What is your RNA-seq material, and What is the reference genome are you using ? ...
written 12 months ago by
tiago211287 • 1.1k
1
vote
2
answers
789
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2
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... Tip: When posting code, use the code sample button to make it easier to read.
"EOF marker is absent" means that your BAM file has been truncated. Did Tophat produce a BAM or a SAM file? How did you convert from sam to bam? ...
written 12 months ago by
tiago211287 • 1.1k
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