User: tiago211287

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tiago211287770
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Posts by tiago211287

<prev • 183 results • page 1 of 19 • next >
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Comment: C: Command or Perl script for changing headers of multiple FASTA files in a specifi
... this is strange to me, it appears that your shell is not exporting the function properly ...
written 11 days ago by tiago211287770
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Comment: C: Command or Perl script for changing headers of multiple FASTA files in a specifi
... can you repeat what you're doing and post a screenshot of your screen here? ...
written 12 days ago by tiago211287770
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Comment: C: Command or Perl script for changing headers of multiple FASTA files in a specifi
... in order to work you must execute this two lines in your terminal: function sedinho () { sed -i "s/^.*\]/>$1/g" $2;} export -f sedinho you dont need to put this inside a file ...
written 12 days ago by tiago211287770
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Answer: A: Command or Perl script for changing headers of multiple FASTA files in a specifi
... First, create a backup of all files before trying this approach. Taking a list of new headers, namely, Headers.txt cat Headers.txt MP.C_AMY2B FP.H_AMY2B in the same order of one fasta files list find . -maxdepth 1 -name "*CDS.fasta" | sort ./Chimpanzee_AMY2B_CDS.fasta ./H ...
written 12 days ago by tiago211287770
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Answer: A: Downloading the latest human genome
... There are many places to download the full human genome, I suggest gencode: [Genome sequence, primary assembly (GRCh38)][1] [1]: https://www.gencodegenes.org/ ...
written 16 days ago by tiago211287770
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Comment: C: Demultiplexing reads with index present in the labels
... Thanks for your comment. I was aware that I would not get all 10%, but I was hopping to recover ~3% from it. Indeed, it seems to me a good idea to look the number and kind of tags are present in the undertermined file. Contrary to what I thought, most of the reads are crap: AAAAAGAA-AAAAAAAA/ ...
written 18 days ago by tiago211287770
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Demultiplexing reads with index present in the labels
... Hello, Earlier I had a problem ( already solved, thanks to the help of Brian Bushnell and Genomax), in which my index reads were not supply in a separated file but in the fastq labels, like this example: @GHAY-HISEQ2:5:2308:2003:1934#TTGCTGGA-ACCAACTG/1;1 NGCATGAACGGCTAAACGAGGGTCCAACTGTCT ...
rnaseq illumina demultiplex index written 18 days ago by tiago211287770
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Comment: C: How to split a fastq file into each corresponding sample.fastq?
... I am glad to contribute. ...
written 19 days ago by tiago211287770
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Comment: C: How to split a fastq file into each corresponding sample.fastq?
... #In theory 25 sequences \/ wc -l test.fq 100 test.fq head -n8 test.fq @GHAY-HISEQ2:5:2308:2003:1934#TTGCTGGA-ACCAACTG/1;1 NGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCT +GHAY-HISEQ2:5:2308:2003:1934#TTGCTGGA-ACCAACTG/1;1 B[[aaeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeee @GHAY- ...
written 19 days ago by tiago211287770
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Comment: C: How to split a fastq file into each corresponding sample.fastq?
... Thank you very much Brian. ...
written 19 days ago by tiago211287770

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