User: James Ashmore

gravatar for James Ashmore
James Ashmore2.6k
Reputation:
2,570
Status:
Trusted
Location:
UK/Edinburgh/MRC Centre for Regenerative Medicine
Twitter:
jma1991
Last seen:
3 weeks, 5 days ago
Joined:
4 years, 6 months ago
Email:
j******@icloud.com

I am a PhD student in Simon Tomlinson's research group at the MRC Centre for Regenerative Medicine. My interests lie in the biology of reprogramming, and developing methods to analyse the reprogramming process.

Posts by James Ashmore

<prev • 295 results • page 1 of 30 • next >
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Comment: C: Software to extract run information from FASTQ headers?
... Thanks Pierre - should be able to convert from JSON to CSV on the fly. ...
written 5 weeks ago by James Ashmore2.6k
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Comment: C: Software to extract run information from FASTQ headers?
... In my case I want to create read group information as explained by [GATK][1] by automating the extraction of run information and creating the read groups for each run/sample. [1]: https://gatkforums.broadinstitute.org/gatk/discussion/6472/read-groups ...
written 5 weeks ago by James Ashmore2.6k
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Comment: C: Software to extract run information from FASTQ headers?
... This isn't homework. There seems to be a surprising number of edge-cases depending on which version of CASAVA was used to convert from BCL to FASTQ format. ...
written 5 weeks ago by James Ashmore2.6k
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Software to extract run information from FASTQ headers?
... Given a directory containing multiple FASTQ files, I would like to retrieve the run information from each file (e.g. flowcell, lane number, index e.t.c) and export the data to a CSV file. Before I write a script to do this myself, is anyone aware of any software which does this already? ...
fastq written 5 weeks ago by James Ashmore2.6k • updated 5 weeks ago by Pierre Lindenbaum118k
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Comment: C: So frustrated compiling necessary packages in a linux system
... +1 for the bioconda suggestion. It really has made my work easier. ...
written 5 months ago by James Ashmore2.6k
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Answer: A: Finding differences in motif enrichment between ATAC peak samples?
... Take a look at the recently released diffTF (https://difftf.readthedocs.io/en/latest/index.html) - it will allow you to infer differential transcription factor binding and motif enrichment. ...
written 5 months ago by James Ashmore2.6k
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Comment: C: Experimental design for RNA-seq time-course with the same start and end points b
... It’s a bit of a specific example but the time-course is for iPS reprogramming - the treatment gets to the end-point faster than the control. The end-point is around 15 days from the beginning. ...
written 5 months ago by James Ashmore2.6k
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Experimental design for RNA-seq time-course with the same start and end points but different treatments
... I have RNA-seq data for a time-course experiment which consists of the same starting and end points but two different conditions: https://postimg.cc/DW0RzJf1 I am unsure how to correctly model the data to answer the following questions: 1. Identify differential genes between each time-point withi ...
rna-seq written 5 months ago by James Ashmore2.6k • updated 4 months ago by Biostar ♦♦ 20
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Comment: C: using bash variable in picard MarkDuplicates command
... Can you paste the error thrown by Picard? ...
written 7 months ago by James Ashmore2.6k
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Comment: C: Analysis of gene expression data
... If you have count data, try following one of the RNA-seq expression tutorials online. Here's a good one to start with: https://f1000research.com/articles/4-1070/v1 ...
written 9 months ago by James Ashmore2.6k

Latest awards to James Ashmore

Popular Question 10 weeks ago, created a question with more than 1,000 views. For Difficulty replicating likelihood ratio test from RNA-seq paper
Popular Question 3 months ago, created a question with more than 1,000 views. For Difficulty replicating likelihood ratio test from RNA-seq paper
Popular Question 3 months ago, created a question with more than 1,000 views. For Difficulty replicating likelihood ratio test from RNA-seq paper
Popular Question 4 months ago, created a question with more than 1,000 views. For Difficulty replicating likelihood ratio test from RNA-seq paper
Appreciated 5 months ago, created a post with more than 5 votes. For A: What "chrUn" stand for in UCSC genome browser web server?
Popular Question 6 months ago, created a question with more than 1,000 views. For Difficulty replicating likelihood ratio test from RNA-seq paper
Popular Question 7 months ago, created a question with more than 1,000 views. For Visualising the number of overlapping peaks in ChIP-seq studies
Good Question 8 months ago, asked a question that was upvoted at least 5 times. For How to handle metadata in bioinformatics pipelines?
Popular Question 8 months ago, created a question with more than 1,000 views. For Visualising the number of overlapping peaks in ChIP-seq studies
Great Question 8 months ago, created a question with more than 5,000 views. For How do I view the height / distribution of ChIP-seq peaks?
Scholar 9 months ago, created an answer that has been accepted. For A: Calculate the number of paired-end reads their middle size is greater than # KB
Scholar 9 months ago, created an answer that has been accepted. For A: Calculate the number of paired-end reads their middle size is greater than # KB
Popular Question 11 months ago, created a question with more than 1,000 views. For Visualising the number of overlapping peaks in ChIP-seq studies
Popular Question 11 months ago, created a question with more than 1,000 views. For Visualising the number of overlapping peaks in ChIP-seq studies
Appreciated 12 months ago, created a post with more than 5 votes. For A: Run multiple fastq.gz files with FastQC and yield single report?
Great Question 14 months ago, created a question with more than 5,000 views. For How do I view the height / distribution of ChIP-seq peaks?
Commentator 14 months ago, created a comment with at least 3 up-votes. For C: Using getopt module to parse and define command line arguments in python
Scholar 15 months ago, created an answer that has been accepted. For A: Calculate the number of paired-end reads their middle size is greater than # KB
Teacher 16 months ago, created an answer with at least 3 up-votes. For A: SRA to fastq
Teacher 16 months ago, created an answer with at least 3 up-votes. For A: SRA to fastq
Good Answer 16 months ago, created an answer that was upvoted at least 5 times. For A: Do I have to check every fastqc to make good quality?
Student 17 months ago, asked a question with at least 3 up-votes. For How do I view the height / distribution of ChIP-seq peaks?
Commentator 17 months ago, created a comment with at least 3 up-votes. For C: Using getopt module to parse and define command line arguments in python
Popular Question 18 months ago, created a question with more than 1,000 views. For Trimmomatic not removing all Nextera adapters or N base calls
Popular Question 18 months ago, created a question with more than 1,000 views. For How to handle metadata in bioinformatics pipelines?

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