User: Antonio R. Franco
Antonio R. Franco • 4.0k
- Reputation:
- 3,950
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- Location:
- Spain. Universidad de Córdoba
- Last seen:
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- Joined:
- 4 years, 4 months ago
- Email:
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Posts by Antonio R. Franco
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... It is sort of hard to understand your questions but if you want to filter your library, take a look to [BBSplit][1]
[1]: http://seqanswers.com/forums/showthread.php?t=41288 ...
written 11 minutes ago by
Antonio R. Franco • 4.0k
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140
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... You cannot convert colorSpace to base format. One incorrect color/transition will propagate such that all the remaining bases will be incorrect upon conversion.
Old versions of Tophat still can map solid sequences. You are running out of alternatives ...
written 3 months ago by
Antonio R. Franco • 4.0k
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149
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Answer:
A: weird number in log2FC
... Try to include dec="," as well in the write.table command, specially if your excel recognize the "," as the decimal digit ...
written 3 months ago by
Antonio R. Franco • 4.0k
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... Wondering why these schemes (Igor's) end with a PCR reaction. If you do a PCR before loading the sequenced into a massive sequencer, you lose the stranded information, so I believe these are sort of confusing
In addition.. I believe Igor's schemes are not right. In the dUTP method it ends with a fr ...
written 3 months ago by
Antonio R. Franco • 4.0k
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... are you in the same directory that samtools is ?
In addition... is the samtools file executable ?. You can see it by running
ls -l
You need to see an "x" at least in the first group of letters after the hyphen
If not, run
chmod +x samtools ...
written 4 months ago by
Antonio R. Franco • 4.0k
1
vote
2
answers
267
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... It is likely you don't have the samtools executable binary in the $PATH. If so, try to run it by using
./samtools
when you are in the directory containing the program ...
written 4 months ago by
Antonio R. Franco • 4.0k
0
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1
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... Shoulders in this graphics are usually compatible with at least two issues
1. Presence of contaminant DNA
2. Poor quality in the sequencing
The point2 can be solved at least in part after a quality trimming. If the shoulder persist after the quality trimming a contamination fo your samples can be ...
written 4 months ago by
Antonio R. Franco • 4.0k
0
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2
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195
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... Of course no. I was simply trying to indicate that if you don't add methylated precursors to your PCR it is impossible to include methylated bases to your DNA. And if you add such as methylated precursors they will not follow the original pattern in any case
...
written 4 months ago by
Antonio R. Franco • 4.0k
0
votes
2
answers
195
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answers
... Neither you add methylated precursors in the PCR to maintain that status. That will be worthless in any case ...
written 4 months ago by
Antonio R. Franco • 4.0k
2
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1
answer
319
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... Why don't you use specialized R packages for time course such as [impulseDE2][1] or [maSigPro][2]
[1]: https://www.bioconductor.org/packages/release/bioc/html/ImpulseDE2.html
[2]: https://www.bioconductor.org/packages/release/bioc/html/maSigPro.html ...
written 4 months ago by
Antonio R. Franco • 4.0k
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For Recommended coverage for methylome analysis
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For Recommended coverage for methylome analysis
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For A: How to find the Genes present in the sequence?
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For A: is there any databse that provides the promoter of a specific gene?
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For A: is there any databse that provides the promoter of a specific gene?
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4 months ago,
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For A: is there any databse that provides the promoter of a specific gene?
Teacher
4 months ago,
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For A: is there any databse that provides the promoter of a specific gene?
Teacher
4 months ago,
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For A: is there any databse that provides the promoter of a specific gene?
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For Recommended coverage for methylome analysis
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For Is qPCR actually required for RNA-Seq validation ?
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For A: is there any databse that provides the promoter of a specific gene?
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For Can the code of a R package be changed? How?
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For A: is there any databse that provides the promoter of a specific gene?
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