User: seta

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seta920
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920
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3 years ago
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Posts by seta

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(Closed) Third-party library preparation kits
... Hi all, Could you please kindly explain to me what means third-party library preparation kits? Sorry if you find the question is basic. Thanks ...
next-gen sequencing written 26 days ago by seta920 • updated 26 days ago by Devon Ryan73k
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Comment: C: Regarding TMM normalization before DEG analysis by DEGseq
... Thank you for your response. As far as I know, TMM is between-sample normalized count (not break the assumption that you mentioned, please correct me if I'm wrong), and, therefore, more suitable for DE analysis, do you agree with me? However, DEseq or edgeR has internally calculated TMM, but unfortu ...
written 11 weeks ago by seta920
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Comment: C: Regarding TMM normalization before DEG analysis by DEGseq
... I asked about DEGseq, it differs from DEseq. ...
written 11 weeks ago by seta920
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Regarding TMM normalization before DEG analysis by DEGseq
... Hi all, Please kindly advise me if using **TMM method for read count normalization and then DEGseq** for identification of differentially expressed genes is the right approach? I never this tool, but as I searched this package shouldn't be used for the analysis because its statistical assumptions a ...
deg analysis degseq tmm written 11 weeks ago by seta920 • updated 11 weeks ago by theobroma221.0k
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Shortest path algorithm in pathway studio
... Hi all, I used pathway studio to construct a biological pathway of some selected DEG obtained from a RNA-Seq analysis. When I selected the shortest path, the tool gave me an error "Find short path algorithm requires exactly 2 entities in selection". But, I saw in papers used this algorithm with sev ...
pathway shortest path algorithm written 3 months ago by seta920
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Comment: C: The same results with various dataset by cufflinks
... Never, not running with the same dataset. I checked all commands again. What should I do?! Actually, the second dataset is those data that two read files of a single paired-end file had various length and I asked about it in this post ([enter link description here][1], and you kindly suggested to r ...
written 5 months ago by seta920
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The same results with various dataset by cufflinks
... Hi everybody, I'm busy with genome-guided transcriptome assembly of some Illumina data from human. I used STAR for read mapping on hg19 and cufflinks for transcriptome assembly. I performed the analysis for two independent datasets, separately (one single end, 36bp and another, paired-end 100 bp). ...
assembly alignment mapping cufflinks written 5 months ago by seta920
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Comment: C: inner and outer genomic coordinates concept
... Thank you very much, h.mon ...
written 5 months ago by seta920
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Comment: C: inner and outer genomic coordinates concept
... Of course, google is the first one. To be honest, I saw the link, but unfortunately didn't get the point of interest. I'm looking for an image for understanding outer and inner genomic mapping coordinate, my problem is the concept of outer and inner, here, not detecting duplicates by mapping. ...
written 5 months ago by seta920
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inner and outer genomic coordinates concept
... Hi everybody, Could you please kindly tell me what is the meaning and concept of outer in the below sentence? so sorry if you found the question is a basic, but I have to ask. Toolkits for aligned reads use the **outer genomic mapping coordinates** of paired-end reads as an indication of identical ...
genome alignment outer coordinate written 5 months ago by seta920 • updated 5 months ago by h.mon9.2k

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