User: bolbolman2000

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Posts by bolbolman2000

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Answer: A: genes counts using htseqcounts
... Thanks for you reply, i did download the gtf file and i run the htseq count a gain using a sam file instead of the .bam aftert i used the samtools to convert it.  but i got the following error   [Malformed SAM line: MRNM == '*' although flag bit &0x0008 cleared", 'line 68 of file sorted_genom ...
written 4.4 years ago by bolbolman20000
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(Closed) genes count in sam file using htseq-count
... I run the htseq-count htseq-count -s no <fileName>.sam genes.gtf > counts.txt And I  got the  following error.. [Malformed SAM line: MRNM == '*' although flag bit &0x0008 cleared", 'line 68 of file sorted_genome_alignments.sam')   [Exception type: ValueError, raised in _HTSeq.pyx:13 ...
page htseq-count rna-seq written 4.4 years ago by bolbolman20000 • updated 4.4 years ago by Michael Dondrup45k
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genes counts using htseqcounts
... Hello All,   i am new to these RNA-seq thing, and i really need help.   i have .bam files that i need to get the genes counts from it to use in the edgeR. i don't have information about the reads but iknow that these .bam files is mapping for read of human. the .bam files belong to two group pat ...
page sequencing written 4.4 years ago by bolbolman20000 • updated 4.4 years ago by Michael Dondrup45k

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