User: Genosa

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Genosa100
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Posts by Genosa

<prev • 25 results • page 1 of 3 • next >
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Comment: C: Plotting graphs using R - how to 'omit' specific values?
... Hi thank you for your reply. However, there are more than 100k 0 values in the data and it's too labour intensive to remove them one by one. Is there another way which I can do this without manipulating data? ...
written 3.8 years ago by Genosa100
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Plotting graphs using R - how to 'omit' specific values?
... Hello, Asking for help from R experts here. I'm totally new in R and I'm just spending lots of time to figure out how to plot scatter plots on R. I have a large data set (>10K rows) which I wish to plot out the data using a scatter plot using this function on the tutorial: http://www.statmethod ...
R written 3.8 years ago by Genosa100 • updated 3.8 years ago by WouterDeCoster44k
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Comment: C: Comparing 2 different sets of RNAseq data - correlation
... Hi Sysbiocoder, Thank you for the useful link. The metioned correlation methods (spearman vs. pearson), which would be the method of choice for comparing 2 database of different sequncing depths (i.e. I also have miseq vs. hiseq data from the same types of cells). I have read online resources bu ...
written 3.8 years ago by Genosa100
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Answer: A: Comparing 2 different sets of RNAseq data - correlation
... Hi Sysbiocoder, Yes, my data is available in Log2 FC or FPKM. May I know which R package I can use? Sorry I am new to R so I'll need some guidance on where to start. ...
written 3.8 years ago by Genosa100
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Comparing 2 different sets of RNAseq data - correlation
... Hi, sorry if this is a really basic question but I'm new to R and bioinformatics. After spending A LOT of time generating my RNAseq data from tuxedo suite, I need to compare 2 datasets generated by different methods. I am not sure what is the best method, but In papers I often see the usage of corr ...
rna-seq written 3.8 years ago by Genosa100 • updated 3.8 years ago by sysbiocoder180
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Comment: C: How to calculate Log2 Fold Change for normalised qPCR data?
... Got it. Thanks very much ...
written 3.8 years ago by Genosa100
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How to calculate Log2 Fold Change for normalised qPCR data?
... Sorry if this is a rather basic question. I am trying to find a good solution on how to calculate fold change for qPCR data normalised to housekeepting genes across different conditions. For example, I have this data: (CT value) Baseline condition Housekeeping gene: 30.7, 30.6, 30.6 Baseline co ...
qpcr written 3.8 years ago by Genosa100 • updated 2.2 years ago by Kevin Blighe66k
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Comment: C: How to transpose data using terminal?
... I am trying to make the data fit the GSEA format ...
written 3.9 years ago by Genosa100
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How to transpose data using terminal?
... Hi, sorry if this is a rather basic terminal question. I have a set of RNAseq output file which is in the current format: gene condition replicate fpkm B2M untreated 0 800 B2M Untreated 1 900 B2M untreated 2 850 ...
rnaseq terminal linux written 3.9 years ago by Genosa100 • updated 3.9 years ago by genomax91k
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Comment: C: How to merge column text files using terminal command?
... Hi Michael, thanks! But the joine data all appear in 1 column rather than separated columns. Is there a way that I can do to separate them? Thank you ! ...
written 3.9 years ago by Genosa100

Latest awards to Genosa

Popular Question 3.8 years ago, created a question with more than 1,000 views. For Single Cell RNA-seq and validation
Popular Question 5.7 years ago, created a question with more than 1,000 views. For Single cell RNAseq - how many cells enough to determine DE?

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