User: alpha09

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alpha0910
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New User
Location:
Norway
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4 days, 16 hours ago
Joined:
4 years, 6 months ago
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Posts by alpha09

<prev • 12 results • page 1 of 2 • next >
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Sub_Population using 10X Loupe browser
... I have scRNA-Seq data. I anticipated different clusters using "Loupe Browser". Now I made my own cluster in loupe, I am interested in finding sub_populations within this cluster. Afterward to find genes associated with these clusters. ...
10x written 12 days ago by alpha0910 • updated 12 days ago by genomax73k
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Pipeline for scRNA-Seq
... I am developing a pipeline for Pituitary of fish by using Single cell transcriptomics. I need help in normalization method. I am also working on "Scater" R package. There is no Spike-In or UMI in my data. ...
R forum next-gen rna-seq written 23 months ago by alpha0910 • updated 23 months ago by Pierre Lindenbaum123k
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Comment: C: RNA Normalization from DESeq to RPKM
... Thank You Devon. ...
written 4.0 years ago by alpha0910
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Answer: A: RNA Normalization from DESeq to RPKM
... Thank You so much. It helped me a lot ...
written 4.0 years ago by alpha0910
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Answer: A: RNA Normalization from DESeq to RPKM
... Total gene read counts were normalized on library size using DESeq method (size factor) Feature_ID M1_1 M1_2 M2_1 M2_2 M3_1 M3_2 M4_1 M4_2 M5_1 M5_2 M6_1 M6_2 M7_1 M7_2 M8_1 M8_2 M9_1 M9_2 M10_1 M10_2 AT1G01010 ...
written 4.0 years ago by alpha0910
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RNA Normalization from DESeq to RPKM
... I have RNA Seq DESeq normalized data.I want to convert it into RPKM. kindly let me know what should I do? Any R package etc. Thank You. ...
R next-gen rna-seq written 4.0 years ago by alpha0910
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Answer: A: Transcription Factor (TF) sequence extraction +/- 2000 of Arabidopsis thaliana
... yes I have names and motif.   ...
written 4.2 years ago by alpha0910
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Comment: C: Differentailly expressed genes of microarray time series data with replicates...
... okay thank you. I got it. ...
written 4.2 years ago by alpha0910
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(Closed) Differentailly expressed genes of microarray time series data with replicates...
... I have micro array data which RMA normalized.I want to extract differentially expressed genes. My data is times series and with replicates.below is the format of my data..(0,4,8,12,16,20) is one day expression and (24,28,32,36,40,44) is 2nd day. Gene_ID LDT0 LDT4 8 12 16 ...
gene R next-gen sequence written 4.2 years ago by alpha0910
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Transcription Factor (TF) sequence extraction +/- 2000 of Arabidopsis thaliana
... I have Arabidopsis thaliana transcription factors and I want to extract sequence  +/- 2000. Is there any tool OR way to extract quickly? Kindly tell me the tool name or any website. I tried different databases of Arabidopsis thaliana. ...
gene R tools next-gen genome written 4.2 years ago by alpha0910

Latest awards to alpha09

Popular Question 23 months ago, created a question with more than 1,000 views. For Gene Network construction
Popular Question 3.9 years ago, created a question with more than 1,000 views. For Gene Network construction
Popular Question 3.9 years ago, created a question with more than 1,000 views. For RNA Normalization from DESeq to RPKM

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