User: orange

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orange30
Reputation:
30
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New User
Location:
Korea, Republic Of
Last seen:
7 months, 3 weeks ago
Joined:
2 years, 4 months ago
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1*********@qq.com

Posts by orange

<prev • 15 results • page 1 of 2 • next >
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minia kmer limit
... hi,everyone     when I use minia 2.0.3 with kmer=141,it fail.But with kmer=127,it work! So minia have kmer limit ? ...
assembly minia written 22 months ago by orange30 • updated 22 months ago by edrezen680
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Answer: A: Assemble short reads based on k-mers
... why not try SOAPdenovo instead of perl ? Did you not aseembly genome sequence before ? ...
written 2.1 years ago by orange30
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Comment: C: Recommendations For Heterozygous Genome Assembly Software
... hi,ScaffMatch support sam and fastq。 ...
written 2.2 years ago by orange30
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check if one unitig is truly circual
... For bacteria assembly with pacbio reads, a unitig is easy at most time. But how to check if it is truly circual ? I tried CA_best_edge_to_GML.py,and an error happen like " Traceback (most recent call last): File "CA_best_edge_to_GML.py", line 51, in id1, lib_id, best5, o1, best3, o3 = l ValueErr ...
celera written 2.3 years ago by orange30 • updated 2.3 years ago by rhall160
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Answer: A: Tool for reducing contigs
... try AHA or PBjelly2 or sspace-longread for scaffoding . ...
written 2.3 years ago by orange30
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Answer: A: how to merge contigs from two assemblers?
... maybe you can try rabbit  ...
written 2.3 years ago by orange30
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Answer: A: how to do fungi diploid genome denovo by pacbio reads
... My firt thought is use SOAP2 to get diploid contigs,then use these contigs and PE reads to correct subreads ,inally use FALCON. ...
written 2.3 years ago by orange30
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fungi diploid genome denovo assembly by pacbio reads
... Hi,everyone.I have a project about 50M fungi diploid denovo assembly.I had assembly 37M fungi haploid with pacbio reads and illuminate and Miseq reads.For haploid genome, I used Ectool and HGAP3. But for diploid genome,I have doubt about how to correct subreads,and use which assembler . And I have ...
assembly written 2.3 years ago by orange30 • updated 17 months ago by Picasa300
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Answer: A: Post Genome Assembly
... when I click "ADD COMMENT " or "ADD REPLY"(color of the button is gray),but it didnot work ..someome tell me why ? ...
written 2.3 years ago by orange30
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Answer: A: Post Genome Assembly
... Contiguator or Medusa seem to be based reference genome,so it is not denovo ... ...
written 2.3 years ago by orange30

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Popular Question 9 months ago, created a question with more than 1,000 views. For fungi diploid genome denovo assembly by pacbio reads

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