User: scottbrouilette

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United Kingdom
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Posts by scottbrouilette

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Comment: C: How much paired end vs single end RNA-seq reads can have influnce on expression
... One simple solution would be to use just single end reads i.e. discard the paired-data. For GEX (as opposed to transcript-level/isoform analysis) there is plenty of data to suggest that 1x50bp data is sufficient.   ...
written 3.9 years ago by scottbrouilette50
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Comment: C: Is checking clustering of technical replicates necessary for RNAseq?
... Technical replicates only provide information about the technical variation - for example, how reproducible is your wet-lab methodology, or your sequencing platform. Assuming you are happy with these aspects there is no need for technical replicates - you then need true biological replicates to incr ...
written 3.9 years ago by scottbrouilette50

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