User: cryptogenomicon

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Posts by cryptogenomicon

<prev • 11 results • page 1 of 2 • next >
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Comment: C: hmm without realigning
... If you want `hmmemit` to sample an alignment, have you tried `hmmemit -a`? This is in the help page and in the documentation: Options controlling what to emit: -a : emit alignment ...
written 4 weeks ago by cryptogenomicon150
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Comment: C: E-value results from hmmsearch are not accurate. [HMMER]
... I agree with that. Looks like the input is a random alignment. The title of the question is misleading. ...
written 16 months ago by cryptogenomicon150
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Comment: C: HMMER: Failed to open target sequence database for reading
... Pfam-A.fasta is a FASTA sequence file. It is not a profile HMM database. (Have you looked at it? If not, look at it.) ...
written 17 months ago by cryptogenomicon150
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Comment: C: HMMER: Failed to open target sequence database for reading
... hmmpress is for HMM files that you're going to search with hmmscan; not for FASTA files that'll be searched with phmmer. ...
written 17 months ago by cryptogenomicon150
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Comment: C: Conflicting consensus sequences from different tools (bug in hmmemit?)
... hmmemit will only output canonical residues (20 aa or 4 nt). ...
written 17 months ago by cryptogenomicon150
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Answer: A: HMMER: Failed to open target sequence database for reading
... It means that your file `HMMER/database/Pfam-A.fasta` can't be opened for reading. It either doesn't exist, or you don't have read permission for it. Also, if you are trying to search a protein sequence against Pfam, you generally want to be using hmmscan or hmmsearch, not phmmer. phmmer is for sea ...
written 17 months ago by cryptogenomicon150
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Answer: A: Conflicting consensus sequences from different tools (bug in hmmemit?)
... Because hmmemit is emitting the maximum likelihood sequence, according to the profile HMM's parameterization. ("majority-rule" in hmmemit means w.r.t. the profile HMM, not w.r.t. the MSA that the HMM was built from. hmmemit takes the HMM as input, not the MSA.) ...
written 17 months ago by cryptogenomicon150
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Comment: C: How to generate a multiple HMM file with HMMER?
... Yes, you can simply concatenate HMMER profile HMM files. ...
written 20 months ago by cryptogenomicon150
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Answer: A: Does hmmsearch look at the reverse complement of the fasta?
... hmmsearch doesn't, but nhmmer (which is meant for DNA/DNA comparison) does. Pretty sure this is documented, too. ...
written 21 months ago by cryptogenomicon150
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Answer: A: Don't get hits on HMMER
... I don't know what you mean by 3' slicing sequences (typo for the 3' splice site consensus?) but: HMMER is intended for identifying statistically significant sequence relationships, as a proxy for sequence homology -- i.e. comparison scores that you wouldn't expect to see by chance in a target seque ...
written 3.8 years ago by cryptogenomicon150

Latest awards to cryptogenomicon

Scholar 17 months ago, created an answer that has been accepted. For A: Conflicting consensus sequences from different tools (bug in hmmemit?)
Teacher 17 months ago, created an answer with at least 3 up-votes. For A: Conflicting consensus sequences from different tools (bug in hmmemit?)

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