User: Louis Kok

gravatar for Louis Kok
Louis Kok10
Reputation:
10
Status:
New User
Location:
Malaysia
Last seen:
1 year, 9 months ago
Joined:
3 years, 1 month ago
Email:
e*********@gmail.com

Posts by Louis Kok

<prev • 31 results • page 1 of 4 • next >
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Hierarchical ranking of gene ontology
... Hi all. I have a question about the gene ontology ranking. For example, GO:0004672 is named as protein kinase activity. From the Tree view, I found that it can be linked to different higher level, such as macromolecule modification, protein metabolic process and others at level 5. I would like class ...
gene ontology written 2.0 years ago by Louis Kok10
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Comment: C: 16S rRNA database for human clinical pathogens?
... Dear Natasha, Many thanks for the information. This is certainly helpful. :) Rgds, Louis ...
written 2.1 years ago by Louis Kok10
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16S rRNA database for human clinical pathogens?
... Hi All, I was wondering if there is any available public 16S rRNA database specific for human bacterial pathogens, other than the generic databases like RDP or SILVA? Thank you. ...
16s pathogens database human written 2.1 years ago by Louis Kok10 • updated 2.1 years ago by natasha.sernova2.9k
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Comment: C: samtools flagstat result is different from samtools view result
... Thanks Dan for the explanation. For the number of reads mapped, would you take in consideration of singletons as well? ...
written 2.2 years ago by Louis Kok10
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samtools flagstat result is different from samtools view result
... Hi All. I wonder if anyone of you have inconsistent mapping result generated by `samtools flagstat` and `samtools view` command? My samtools flagstat output is as below: 3217 + 0 in total (QC-passed reads + QC-failed reads) 0 + 0 duplicates 2788 + 0 mapped (86.66%:nan%) 393 + 0 rea ...
samtools written 2.2 years ago by Louis Kok10 • updated 2.2 years ago by Dan D6.4k
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Comment: C: Downloading the raw 16S amplicon data for all saliva samples from the Human Micr
... Hi. Do you know if there is any particular online resource you would like to download from? ...
written 2.2 years ago by Louis Kok10
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Comment: C: How to creat a phylogenetic tree for SPECIES?
... Try using the protein sequences of housekeeping genes, such as DNA/RNA polymerase genes. Those genes are usually conserved and are able to distinguish the ancestral relationship for the species you study. Unless, you are looking particularly a gene that you are interested. I have heard that MrBayes ...
written 2.2 years ago by Louis Kok10
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Comment: C: TargetP Error: zero output generated
... I successfully run it in Ubuntu guest OS in my Windows 7 laptop even thought I still could not find the actual reason. ...
written 2.2 years ago by Louis Kok10
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Comment: C: How to validate genes identified computationally
... If you have identified the causal gene, you can probably design the primers to flank the regions of the gene. Make sure to check for primer specificity to avoid unwanted regions to be amplified from the genome. After that, the amplified PCR products can be validated using targeted sequencing by Sang ...
written 2.2 years ago by Louis Kok10
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Comment: C: How to creat a phylogenetic tree for SPECIES?
... You can practically use any type of DNA sequences to build phylogenetics. Let me know if this is what you are asking for. ...
written 2.2 years ago by Louis Kok10

Latest awards to Louis Kok

Popular Question 2.0 years ago, created a question with more than 1,000 views. For Convert alignment in Fasta/Clustal format to SAM/BAM file
Popular Question 2.1 years ago, created a question with more than 1,000 views. For Convert alignment in Fasta/Clustal format to SAM/BAM file

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