User: Manuel Landesfeind

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Göttingen, Germany
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Posts by Manuel Landesfeind

<prev • 117 results • page 1 of 12 • next >
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Answer: A: How to build a bioinformatics server
... If you have already an R package for the analysis task available and you just want to have some web-interface to make it accessible, a quick alternative might be the development of a [Shiny app][1]. Anyway, for larger and more complex projects, I recommend liux.bio's answer [1]: https://shiny.r ...
written 12 weeks ago by Manuel Landesfeind1.1k
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Comment: C: Inferring genotype based on RNA sequnces
... Some time ago I have done a comparison of WES and RNA-Seq variant calling in a setting with more samples (92) but different software (GATK vs. Samtools) for the calling (which might introduce additional bias), but I confirm that the overlap between both methods is rather low. You may want to have a ...
written 3 months ago by Manuel Landesfeind1.1k
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Comment: C: Appropriate methodologies of comparing whole exome sequencing data between circu
... I agree, in that case you should not use tumor as normal. But than I would still go forward with calling mutations on all samples and removing everything found in population databases with decent frequency. ...
written 5 months ago by Manuel Landesfeind1.1k
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Comment: C: Appropriate methodologies of comparing whole exome sequencing data between circu
... About the somatic variants: you can annotate with ExAC (or other population databases) to remove "putative germline" from your analysis. There are plenty of suggestions on Biostars already. Second, depending on your scientific question, it might be sufficient to use the "tumor" as "normal" because y ...
written 5 months ago by Manuel Landesfeind1.1k
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Comment: C: what is the difference between GRCh37 and hg19 ?
... The term "GRCh38" refers to the genomic assembly. The ".78" and ".91" refer to the version of gene annotation provided by Ensembl, i.e. where do introns/exons/etc start and end. To my knowledge, the genomic sequence for "GRCh38.78" and "GRCh38.91" should be identical and only the gene annotation sho ...
written 5 months ago by Manuel Landesfeind1.1k
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Answer: A: Another statistical model to estimate gene/transcript expression
... @Asaf already mentioned that the topic is well-studied and I agree that little improvements will have little impact. In particular if you have to demonstrate economic impact in your proposal. Industry likes de facto standards and well-established and often-used methods. I work in a CRO with all ki ...
written 12 months ago by Manuel Landesfeind1.1k
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Comment: C: Somatic Mutation Identification for Tumor without Normal
... Thats interesting... for some reason I thought it would be hg38... don't know why.. ...
written 16 months ago by Manuel Landesfeind1.1k
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Comment: C: Somatic Mutation Identification for Tumor without Normal
... What can be discussed is the threshold you use: when do you consider a variant as putative germline? 1%, 10%, ... allelic frequency? In any population or overall? PS: If you figure out a good threshold or some literature, please share here because it will save me some time to evaluate this by mysel ...
written 16 months ago by Manuel Landesfeind1.1k
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Comment: C: Somatic Mutation Identification for Tumor without Normal
... I absolutely agree with Titus that gnomAD and ExAC are key databases. We use 1000G, HapMap and others because our established pipeline uses hg19 genome assembly. Moving to hg38 and corresponding databases is in progress and I plan to use gnomAD and ExAC too. ...
written 16 months ago by Manuel Landesfeind1.1k
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Answer: A: Somatic Mutation Identification for Tumor without Normal
... For me the number of mutations in your tumors seems suspiciously high. We do a very similar approach in removing potential germline mutations from tumors lacking matched normal samples (using different annotation databases and no internal normal-pool). In our models we usually retain between 500 an ...
written 16 months ago by Manuel Landesfeind1.1k

Latest awards to Manuel Landesfeind

Popular Question 12 weeks ago, created a question with more than 1,000 views. For Influence of RNA-seq read splitting on the BAQ calculation in samtools mpileup
Teacher 10 months ago, created an answer with at least 3 up-votes. For A: How to cache reads?
Appreciated 12 months ago, created a post with more than 5 votes. For C: Stop to make GUI with Java .... Use Qt 5 !!
Appreciated 15 months ago, created a post with more than 5 votes. For C: Stop to make GUI with Java .... Use Qt 5 !!
Teacher 15 months ago, created an answer with at least 3 up-votes. For A: How to cache reads?
Guru 17 months ago, received more than 100 upvotes.
Teacher 17 months ago, created an answer with at least 3 up-votes. For A: How to cache reads?
Centurion 18 months ago, created 100 posts.
Teacher 23 months ago, created an answer with at least 3 up-votes. For A: How to cache reads?
Scholar 23 months ago, created an answer that has been accepted. For A: Changing the header of a VCF file and the field correspondingly
Teacher 2.1 years ago, created an answer with at least 3 up-votes. For A: How to cache reads?
Good Answer 2.1 years ago, created an answer that was upvoted at least 5 times. For A: Splice aware aligner - what does it mean?
Scholar 2.2 years ago, created an answer that has been accepted. For A: Changing the header of a VCF file and the field correspondingly
Scholar 2.3 years ago, created an answer that has been accepted. For A: Changing the header of a VCF file and the field correspondingly
Teacher 2.3 years ago, created an answer with at least 3 up-votes. For A: How to cache reads?
Teacher 2.3 years ago, created an answer with at least 3 up-votes. For A: How to cache reads?
Appreciated 2.6 years ago, created a post with more than 5 votes. For C: Stop to make GUI with Java .... Use Qt 5 !!
Scholar 2.6 years ago, created an answer that has been accepted. For A: Changing the header of a VCF file and the field correspondingly
Teacher 2.6 years ago, created an answer with at least 3 up-votes. For A: How to cache reads?
Voter 2.6 years ago, voted more than 100 times.
Commentator 2.7 years ago, created a comment with at least 3 up-votes. For C: How to deal with many uncharacterized protein in the blastx results?
Scholar 2.7 years ago, created an answer that has been accepted. For A: Changing the header of a VCF file and the field correspondingly
Teacher 2.7 years ago, created an answer with at least 3 up-votes. For A: How to cache reads?
Commentator 2.8 years ago, created a comment with at least 3 up-votes. For C: How to deal with many uncharacterized protein in the blastx results?
Appreciated 2.8 years ago, created a post with more than 5 votes. For C: Stop to make GUI with Java .... Use Qt 5 !!

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