User: yueli7
yueli7 • 50
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Posts by yueli7
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... Seurat allows you to easily explore QC metrics and filter cells based on any user-defined criteria. ...
written 2 days ago by
yueli7 • 50
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... Hello, ATpoint,
Thank you for your response！
There is no suggestion in the tutorial:
https://satijalab.org/seurat/v3.1/pbmc3k_tutorial.html
best,
Yue ...
written 5 days ago by
yueli7 • 50
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... Hello, Anyone,
This command in Seurat package means that :
We filter cells that have over 2,500 or less than 200, >5% mitochondrial counts.
If I worked on human, how many unique feature counts we have to filter?
Thanks in advance for great help!
Best,
Yue
pbmc <- subset(pbmc, subs ...
written 5 days ago by
yueli7 • 50
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... Hello, jared.andrews07,
Thank you so much for your response!
Thank you again!
Best,
Yue ...
written 8 days ago by
yueli7 • 50
1
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... Hello, Kevin Blighe,
Thank you so much for your response!
Thank you so much for your useful linkage.
I really appreciated!
Thank you again!
Best,
Yue ...
written 10 days ago by
yueli7 • 50
0
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1
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115
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... Hello, Medhat,
Already change the title.
Thank you!
Best,
Yue ...
written 11 days ago by
yueli7 • 50
0
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... Hello,
Does anyone know what is the mean of the following figure?
I know it is heatmap, what is the meaning of four blocks?
It comes from a single-cell RNA-seq, Seurat package.
Thanks in advance for any help!
Best,
Yue
...
written 11 days ago by
yueli7 • 50
• updated
10 days ago by
jared.andrews07 • 3.9k
2
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100
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... Hello,
Anyone now what is the mean of following figure?
I know it is the PC_1, what is the number in x-axis?
It comes from single-cell RNA-seq Seurat package.
Thanks in advance for any help!
Best,
Yue
![enter image description here][1]
[1]: https://ibb.co/3k4KM2G ...
written 11 days ago by
yueli7 • 50
• updated
11 days ago by
Kevin Blighe ♦ 51k
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80
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Comment:
C: GSEA Must be finite.
...
Just add 0.8 in each number (column or row). 0.8 is a pseudocount, just not let any number to be 0. ...
written 17 days ago by
yueli7 • 50
0
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80
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Comment:
C: pseudocount of dataset
... Just add 0.8 in each number (column or row). 0.8 is a pseudocount, just not let any number to be 0. ...
written 17 days ago by
yueli7 • 50
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For DESeq pvalue>0.05 qvalue=1
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