User: Sujata

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Sujata0
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Posts by Sujata

<prev • 13 results • page 1 of 2 • next >
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PCR duplicates and sequence duplicates
... How to distinguish between PCR duplicates and sequence duplicates after sequencing and which tool is good for removing them? ...
preprocessing tools pcr duplicates written 1 day ago by Sujata0 • updated 23 hours ago by lieven.sterck6.9k
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Comment: A: Single-end sequencing versus paired-end
... [Single versus Paired-end][1] So, if a single-end reads spans across two exons, will it not be possible to figure out the same here? [1]: https://www.ncbi.nlm.nih.gov/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&p=PMC3&id=3688553_pgen.100359 ...
written 3 days ago by Sujata0
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Comment: C: Single-end sequencing versus paired-end
... Thank you for your reply @genomax. However, I did not understand your statement "Two reads coming from the fragment being sequenced would align much farther apart than just the size of the insert, if the fragment contains a splice site". Still, I am a bit confused about when to use single and paired ...
written 4 days ago by Sujata0
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Forum: Single-end sequencing versus paired-end
... Single run is used for counting or differential gene expression, while paired-run is used for splice variants, mutations etc. Why? ...
assembly forum sequencing snp single versus paired written 4 days ago by Sujata0
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Comment: C: Library Prep for NGS
... I agree to some extent but what about my specific question as to : Depth and coverage are decided at the start of the experimental design but exactly at which stage of library prep is it implemented? ...
written 11 weeks ago by Sujata0
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(Closed) Library Prep for NGS
... The basic steps of a NGS library are as follows" 1.Sample extraction 2. DNA Fragmentation 3. End Repair and Adapter Ligation 4. Size Selection (varies according to different platforms chosen) 5. Library Amplification (by PCR) 6. Library QC (by qPCR, fluorometry, spectrophotometry and gel electrophor ...
next-gen library sequencing written 11 weeks ago by Sujata0
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Comment: C: Whole genome sequencing or whole exome sequencing- which is better and why?
... I am starting from basics and that is why my questions may not make sense. I am trying to understand how different technologies work and questions like... (i) How does TPS work? How is it different? coverage versus depth? ...
written 3 months ago by Sujata0
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Comment: C: Whole genome sequencing or whole exome sequencing- which is better and why?
... How is library preparation done for TPS? how do TPS works? Please provide animation or article. ...
written 3 months ago by Sujata0
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Comment: C: Whole genome sequencing or whole exome sequencing- which is better and why?
... Where does Targeted Panel sequencing come in terms of library preparation in sequencing and which sequencing platform would be ideal for finding mutations in cancer? ...
written 3 months ago by Sujata0
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Comment: C: Whole genome sequencing or whole exome sequencing- which is better and why?
... Right I will post it in questions section. Thank you for the link ...
written 3 months ago by Sujata0

Latest awards to Sujata

Popular Question 3.4 years ago, created a question with more than 1,000 views. For 2d- heatmap for RNA-Seq study
Autobiographer 8.7 years ago, has more than 80 characters in the information field of the user's profile.

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