User: Sujata

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Sujata0
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Posts by Sujata

<prev • 9 results • page 1 of 1 • next >
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Comment: C: Library Prep for NGS
... I agree to some extent but what about my specific question as to : Depth and coverage are decided at the start of the experimental design but exactly at which stage of library prep is it implemented? ...
written 4 days ago by Sujata0
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(Closed) Library Prep for NGS
... The basic steps of a NGS library are as follows" 1.Sample extraction 2. DNA Fragmentation 3. End Repair and Adapter Ligation 4. Size Selection (varies according to different platforms chosen) 5. Library Amplification (by PCR) 6. Library QC (by qPCR, fluorometry, spectrophotometry and gel electrophor ...
next-gen library sequencing written 5 days ago by Sujata0
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Comment: C: Whole genome sequencing or whole exome sequencing- which is better and why?
... I am starting from basics and that is why my questions may not make sense. I am trying to understand how different technologies work and questions like... (i) How does TPS work? How is it different? coverage versus depth? ...
written 18 days ago by Sujata0
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Comment: C: Whole genome sequencing or whole exome sequencing- which is better and why?
... How is library preparation done for TPS? how do TPS works? Please provide animation or article. ...
written 18 days ago by Sujata0
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Comment: C: Whole genome sequencing or whole exome sequencing- which is better and why?
... Where does Targeted Panel sequencing come in terms of library preparation in sequencing and which sequencing platform would be ideal for finding mutations in cancer? ...
written 19 days ago by Sujata0
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Comment: C: Whole genome sequencing or whole exome sequencing- which is better and why?
... Right I will post it in questions section. Thank you for the link ...
written 19 days ago by Sujata0
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Whole genome sequencing or whole exome sequencing- which is better and why?
... if genomic DNA is extracted from tissues, and the goal is to find variants such as SNP, indels, CNVs-which is the preferred method- whole exome sequencing or whole genome sequencing and why? Under what cases can we use RNA sequencing to find variants? ...
exome whole genome sequencing rna-seq sequencing written 19 days ago by Sujata0 • updated 19 days ago by WouterDeCoster42k
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Comment: C: 2d- heatmap for RNA-Seq study
... I tried using CummeRbund but it doesn't give 2d- heatmaps with dendograms on both sides ...
written 3.2 years ago by Sujata0
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2d- heatmap for RNA-Seq study
... I have two samples and each has biological replicates. I have run Cufflinks, cuffcompare, and then cuffdiff on it. How do I draw a 2d-heatmap from the cuffdiff or cufflinks output? I would like to use R for it. Any suggestions? ...
R next-gen rna-seq written 3.2 years ago by Sujata0 • updated 3.2 years ago by EagleEye6.5k

Latest awards to Sujata

Popular Question 3.2 years ago, created a question with more than 1,000 views. For 2d- heatmap for RNA-Seq study
Autobiographer 8.5 years ago, has more than 80 characters in the information field of the user's profile.

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