User: mrs.hope

gravatar for mrs.hope
mrs.hope10
Reputation:
10
Status:
New User
Location:
Russian Federation
Last seen:
1 year, 6 months ago
Joined:
3 years, 6 months ago
Email:
m*******@yandex.ru

Posts by mrs.hope

<prev • 5 results • page 1 of 1 • next >
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Is the consistency of the NGS library size important?
... Hello everyone! I have a general question about the importance of keeping the library fragment size consistent between samples. All my libraries have fragments ranging from 200 to 1000 bp according to the Bioanalyzer, which is fine for the Illumina paired-end sequencing. However, some of the librari ...
next-gen library prep rna-seq sequencing written 18 months ago by mrs.hope10
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How to create a bedGraph file for broadPeak peak caller?
... I am trying to use broadPeak package (http://jordan.biology.gatech.edu/page/software/broadpeak/) on my ChIP-seq data. As an input file I should provide "...The input file of the sorted ChIP-seq read-mapping profile in the genome needs to be in bedGraph format. The four tab-delimited columns are chro ...
bedgraph broadpeak chip-seq peak calling written 3.1 years ago by mrs.hope10 • updated 2.2 years ago by 5694977350
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Comment: C: Remove mitochondrial reads from BAM files
... this approach worked the best for me. thanks! ...
written 3.1 years ago by mrs.hope10
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How to get rid of adapter contamination in the middle of the read
... Hello! I have performed my first run on NextSeq 500 machine. I have ChIP-seq samples, pair-end reads, 40 bp long each. I am getting very weird FastQC results for my fastq files. For the first read I consistently have k-mers enrichment in the middle of the read (see pic attached), where k-mers corres ...
illumina nextseq500 adapter contamination written 3.2 years ago by mrs.hope10
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(Closed) Immunostaining non-hisstones protein in histone extracts
... Hi! I have been extraction histones using 0.8M HCl. I have used acid-soluble fraction for SDS-PAGE and subsequent WB. I found that I can actually stain extract for other nuclear proteins - for example histone-modifying enzymes (H3K4 methyltransferases, RING1B). I wonder why I can see these proteins ...

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