User: bioinforesearchquestions

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Posts by bioinforesearchquestions

<prev • 285 results • page 1 of 29 • next >
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Comment: C: How to get differential gene expression within paired samples using EdgeR or DES
... Hi [Kevin][1] I have a very similar situation and also interested in getting the below kind of comparison for my dataset. > In order to compare, Patient51 N vs Patient51 T, what design and contrast should be selected? You are correct that the p-value comes from a 1 versus 1 comparison should ...
written 9 weeks ago by bioinforesearchquestions280
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Comment: C: How to Normalize the effect between group in bulk RNAseq?
... Thanks for your help, ATpoint. Sure, I will try to contact a statistician. ...
written 3 months ago by bioinforesearchquestions280
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Comment: C: How to Normalize the effect between group in bulk RNAseq?
... Hi ATpoint, I have also another kind of analysis. Total 24 samples (12samples from population1 and 12 samples from population2). I separated the pop1 samples in separate dataframe and similarly the corresponding sampleinfo to a separate dataframe. Then I used following ...
written 3 months ago by bioinforesearchquestions280
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Comment: C: How to Normalize the effect between group in bulk RNAseq?
... I have made few changes on my original post. As you mentioned I read the DESeq2 manual page 42. I have 2 populations in 4 conditions (tumor_drug,tumor alone, wt_drug, wt) as mentioned above. So I prepared the sample metadata with the below details. **Is my sample metadata in the correct format for ...
written 3 months ago by bioinforesearchquestions280
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Comment: C: How to Normalize the effect between group in bulk RNAseq?
... My collaborator gave me a list of 40 genes and asked me to do the comparisons on those genes. But I told him that I will do on the entire gene list and filter those 40 genes. Is there a way to do only on those 40 genes? For instance, some set of genes are highly expressed in both Tumor-Drug-Condit ...
written 3 months ago by bioinforesearchquestions280
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Comment: C: How to Normalize the effect between group in bulk RNAseq?
... Sure, I will update myposts. ...
written 3 months ago by bioinforesearchquestions280
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Comment: C: How to Normalize the effect between group in bulk RNAseq?
... Yes, in some of the posts, I didn't accept the answers. I thought it didn't completely address my query. Hereafter in future I will make sure of it. Thanks genomax. ...
written 3 months ago by bioinforesearchquestions280
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Comment: C: How to Normalize the effect between group in bulk RNAseq?
... First I posted this question and then I checked my previous query. Sorry genomax you people are really great in sparing your valuable time and helping us with our queries. ...
written 3 months ago by bioinforesearchquestions280
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Comment: C: Tutorials: Trajectory analysis using Velocity
... I was using jeff works blog for my initial data analysis. Kallistobus is a new reference. Thanks, genomax for the links. I will go over them. ...
written 3 months ago by bioinforesearchquestions280
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How to Normalize the effect between group in bulk RNAseq?
... Hi guys, I have a bulk RNAseq dataset with 24 samples under 4 groups/conditions. I would like - Group/Condition1 : With tumor and treated with a drug (**T-D-P1 and T-D-P2**)(two populations each with three replicates) - Group/Condition2 : With tumor and no treatment (**T-S-P1 and T-S-P2**)(two p ...
drug treatment rna-seq normalize written 3 months ago by bioinforesearchquestions280

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