User: 1234anjalianjali1234

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India
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3 years, 7 months ago
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Posts by 1234anjalianjali1234

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Comment: C: denovo transcriptome assembly
... two kind of samples with triplicates so its n=3. Thankyou ...
written 7 days ago by 1234anjalianjali123430
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Comment: C: denovo transcriptome assembly
... Same plant species producing two physiologically distinct roots. Two samples from two different plants of same species. ...
written 7 days ago by 1234anjalianjali123430
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denovo transcriptome assembly
... hellow all, I want to check the differences between two physiologically distinct roots in a plant (do not have reference genome). I have done RNA sequencing using novaseq, and want to do differential gene expression analysis, but i am confused in denovo transcriptome assembly. Do I need to have s ...
assembly rna-seq written 7 days ago by 1234anjalianjali123430 • updated 6 days ago by kristoffer.vittingseerup1.8k
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Comment: C: Bcl2fastq conversion according to samples (demultiplexing)
... Yes you are right, I will look into them. Thankyou ...
written 8 weeks ago by 1234anjalianjali123430
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Comment: C: Bcl2fastq conversion according to samples (demultiplexing)
... sorry, but i have confirmed that my data is from Illumina GAIIx sytem. IEM is not compatible with GAIIx. Thankyou ...
written 8 weeks ago by 1234anjalianjali123430
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Comment: C: Bcl2fastq conversion according to samples (demultiplexing)
... output of > ls -1 *.fastq.gz Undetermined_S0_L001_I1_001.fastq.gz Undetermined_S0_L001_R1_001.fastq.gz Undetermined_S0_L001_R2_001.fastq.gz Undetermined_S0_L002_I1_001.fastq.gz Undetermined_S0_L002_R1_001.fastq.gz Undetermined_S0_L002_R2_001.fastq.gz Undetermined_S ...
written 8 weeks ago by 1234anjalianjali123430
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Comment: C: Bcl2fastq conversion according to samples (demultiplexing)
... I have updated my SampleSheet.csv file: [Header],,,, Date,2019-04-12,,, Workflow,GenerateFASTQ,,, Application,FASTQOnly,,, Assay,TruSeq,,, Description,,,, Chemistry,,,, ,,,, ,,,, [Reads],,,, 72,,,, 72,,,, ,,,, ,,,, [Data],,,, Lane,Samp ...
written 8 weeks ago by 1234anjalianjali123430
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Comment: C: Bcl2fastq conversion according to samples (demultiplexing)
... we have our sample in 3 different lanes for e.g. 0_DPI_INFECTED samples are in 3 different lane (lane- 2,6 and 7). ...
written 8 weeks ago by 1234anjalianjali123430
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Comment: C: Bcl2fastq conversion according to samples (demultiplexing)
... Yes, you are correct. This is what i want to do for each sample. Sorry, i was not very much clear in my question. ...
written 8 weeks ago by 1234anjalianjali123430
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Comment: C: Bcl2fastq conversion according to samples (demultiplexing)
... Sorry if it doesn't sound right. thank you for the advise I will try this. But our samples were not pooled, we used biological triplicate for each sample. e.g. 0_DPI_INFECTED have 3 biological replicate in 3 different lane (lane- 2,6 and 7). Sorry, I am not good in technical terms. ...
written 8 weeks ago by 1234anjalianjali123430

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Popular Question 7 months ago, created a question with more than 1,000 views. For design matrix for dge
Popular Question 7 months ago, created a question with more than 1,000 views. For design matrix for dge

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