User: loly.pearl86

gravatar for loly.pearl86
loly.pearl8630
Reputation:
30
Status:
New User
Location:
Australia
Last seen:
2 years, 4 months ago
Joined:
4 years, 8 months ago
Email:
l***********@gmail.com

Posts by loly.pearl86

<prev • 31 results • page 1 of 4 • next >
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(Closed) Poor PCR product
... Hi everyone, Actually, i am working on gene expression project and aimed to use RT-PCR and NGS methods. The NGS method work well BUT the PCR product for RT-PCR method was poor. I designed the primers using primer-BLAST and then run PCR to check the presence and absence of the product. I run the g ...
gene written 2.4 years ago by loly.pearl8630
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Comment: C: how can i count the total exon in the sequence structure?
... Where can i write the script? ...
written 3.2 years ago by loly.pearl8630
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Comment: C: how can i count the total exon in the sequence structure?
... Its Sea anemone and i have the Gene and protein sequences and i want to analyse the genomic organisation incluing the total exon/intron, central regioun and functional protien domain ...
written 3.2 years ago by loly.pearl8630
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how can i count the total exon in the sequence structure?
... Hi, Can someone help me to know how can i count the number of exon within the sequence of target genes? In general i would like to visualized the whole sequence structure. I use SMART but its only show the functional domains within the sequences and i want to see the number of exons and UTRs re ...
gene rna-seq written 3.2 years ago by loly.pearl8630
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BLAST fasta format to other fasta
... Hi, Can someone help me how can i BLAST my fasta sequences candidate to other specific species which is in fasta format (Trinity.fasta sequences) Both of them on my computer (the fast for the other species and my candidate fasta.sequences) I know how to used BLAST in NCBI but it take long time a ...
rna-seq blast written 3.3 years ago by loly.pearl8630 • updated 3.3 years ago by h.mon30k
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Gene set function
... Hi Can someone suggest where can i run GSEA ,i used to use trinity scripts but i dont know recently its doesn't work well and give me errors. So i dont want loss more time trying to fix the error if some one can suggest other way i will be thanks I have my DGEs and annotation results files gener ...
next-gen rna-seq written 3.6 years ago by loly.pearl8630
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Novel genes identify
... Hi, Can someone explain to me how can i identify the novel genes in my set of gene of non-model organism ? is the novel gene ,the sequences which does not match similarity with other organism when functional annotation run ?? So all sequences with no BLAST results are novels?? Thanks ...
next-gen written 3.6 years ago by loly.pearl8630 • updated 3.6 years ago by bongok40
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Comment: C: Gene function KEGG and GSEA
... Thanks for your answer. I mean that the group of gene of GO terms some of them related to protection role in the cell and other reasonable about metabolism ...etc of different function ..so how can i summaries my set of gene, i mean are there any way to do them instead of read and identify them ind ...
written 3.7 years ago by loly.pearl8630
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Gene function KEGG and GSEA
... Hi everyone, My question here is about KEGG pathway , 1) When i do DGE to understand the function/role of specific gene and followed by GSEA, Do i still need to perform the KEGG pathway ? or GSEA its enough to identify the gene function ?? 2) I got GSEA results and its iluustrated list of GO term ...
gene written 3.7 years ago by loly.pearl8630
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Comment: C: protein and protein like
... Thanks alot for your answer ...
written 3.7 years ago by loly.pearl8630

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Popular Question 3.2 years ago, created a question with more than 1,000 views. For Trinity, longest contigs
Popular Question 3.2 years ago, created a question with more than 1,000 views. For what is the biological replications

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