User: apuhegde

gravatar for apuhegde
apuhegde20
Reputation:
20
Status:
New User
Location:
United States
Last seen:
1 day, 23 hours ago
Joined:
2 years ago
Email:
a*******@gmail.com

Posts by apuhegde

<prev • 9 results • page 1 of 1 • next >
0
votes
1
answer
185
views
1
answer
COSMIC-like database for mouse sequencing data
... I have DNA sequencing data on murine samples, which I want to run through a database like COSMIC to annotate cancer-relevant mutations. I see this question has already been asked here: http://seqanswers.com/forums/showthread.php?t=70729&utm_source=feedburner&utm_medium=feed&utm_campai ...
mouse cancer cosmic mutations homologous written 8 weeks ago by apuhegde20 • updated 7 weeks ago by EagleEye4.8k
0
votes
0
answers
1.1k
views
0
answers
Comment: C: SNPeff result interpretation
... Hello, this is almost 3 years late, but were you able to interpret the meaning of this finally? Please do share what you learnt, if you did. Thanks! ...
written 8 weeks ago by apuhegde20
0
votes
0
answers
173
views
0
answers
Multiple biopsy issue in MutSig and GISTIC tools
... Hi all, I need to perform GISTIC and MutSig analyses on a cohort of samples. Some of these samples have multiple biopsies taken at both A) same time point, B) different time points. I was wondering what the best way is to go about this situation for the two analyses I mentioned: Specifically: 1) ...
gistic multiple samples multiple biopsies mutsig written 10 weeks ago by apuhegde20
0
votes
3
answers
866
views
3
answers
Comment: C: Snipsift synonymous variant extraction
... Check my reply to basalganglia. ...
written 4 months ago by apuhegde20
0
votes
3
answers
866
views
3
answers
Comment: C: Snipsift synonymous variant extraction
... Bash uses "!" expression in its history substitution command. So it confuses your command with history substitution function and throws an error that it didn't find such a command in its history. Try temporarily turning off history substitution feature and turning it back on when you're done with y ...
written 4 months ago by apuhegde20
0
votes
0
answers
1.1k
views
0
answers
Comment: C: How to do unsupervised clustering using copy number variation data?
... Could you please elaborate a little on "The key is to get a vector representation of the samples" and "they represented each tumor with a vector"? Thanks. ...
written 4 months ago by apuhegde20
0
votes
2
answers
3.0k
views
2
answers
Comment: C: How to use MutSigCV correctly
... Hello, I stumbled across this thread while searching for something similar. So if I understood you correctly, after SNP annotation (I'm using Haplotype Caller), I'm left with a VCF file, from which I need to remove all the annotated SNPs. This leaves me with only un-annotated variants (which are t ...
written 6 months ago by apuhegde20
0
votes
3
answers
1.4k
views
3
answers
Answer: A: how to combine RNA seq data from 4 lanes
... If I had paired-end data, I would combine all the forward read files together, and all the reverse read files together, for all lanes and all flow cells, am I right (assuming there are no batch effects due to lanes and flow cells)? One concern I have is the index (barcode) sequence for the differen ...
written 6 months ago by apuhegde20
6
votes
2
answers
535
views
2
answers
Update on the best pipeline for sequencing?
... It's been 6.4 years since this question was asked last: https://www.biostars.org/p/1268/ I have this same question but I see that 6.4 years ago people were of the opinion that a lot of the best practices were still under development and included quite a bit of of trial and error. I'm assuming thin ...
exome next-gen whole genome rna-seq sequencing written 13 months ago by apuhegde20 • updated 13 months ago by vchris_ngs4.1k

Latest awards to apuhegde

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 839 users visited in the last hour