User: micro32uvas

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micro32uvas10
Reputation:
10
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New User
Location:
Pakistan
Last seen:
6 days, 14 hours ago
Joined:
3 years, 2 months ago
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m**********@gmail.com

Posts by micro32uvas

<prev • 54 results • page 1 of 6 • next >
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Beauti and Beast
... Hello, I am working on bayesian analysis. I run BEAUTI and BEAST for MCMC runs for BSP analysis. I need to set 32% nucleotide substitution per million years but i cannot figure it out well. Can anyone help me out in the result interpretation. Ainnie ...
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Arlequin interpretation for demography and molecular indices
... Hi there, I used Arlequin for a Molecular indices, demographic expansions, comparing the pairwise distances and pairwise Fst between 5 groups. Now that i got the results, I cannot figure out how to interpret them as well as if my deduced results are right. Can anyone help me in reading the arlequi ...
pairwisefst demographic expansion arlequin written 5 weeks ago by micro32uvas10
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Comment: C: NovoPlasty is producing identical Assemblies
... Yeah and to combat this issue, I went with Sanger for the confirmation. Turned out there was read depth problem at some locations, in case of multiple assemblies. They didn't vary much but few of them made multiple. Ruled the problem out by Sanger. ...
written 4 months ago by micro32uvas10
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Mitochondrial genomic analysis by PCoA, PCA and Arlequin
... Hello everyone, I have been working on the mitochondrial genome but it is not my expert field. I have extracted complete mitochondria from my whole genome sequenced samples. So far, I have used MEGA, DNASP and other related software. But I want to use Arlequin for population analysis but cannot f ...
organelle genome pca pcoa arlequin written 4 months ago by micro32uvas10
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NovoPlasty is producing identical Assemblies
... Hello, I have been extracting the mitochondria from my whole genome sequence data. This one sample, produced 3 assemblies, 2 of them are identical and the third one is close to the validated sequence (I did sanger to compare) It is seed and extend based and I cannot understand why it produced exa ...
assembly novoplasty mitochondria written 6 months ago by micro32uvas10 • updated 4 months ago by Biostar ♦♦ 20
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(Closed) Diff gene expression for knock out gene on RNA Seq data
... Hi there, I have got this RNA seq data in .csv format. doe degs. I need to find the knock out gene effect on this data. Can anyone help in a simplified manner? Awaiting your response. Ainnie NC1_length NC1_expected_count NC1_FPKM NC1_Symbol NC2_length NC2_expected_count NC2_FPKM NC2_Symbol ...
R degs knockout gene rna-seq written 10 months ago by micro32uvas10 • updated 9 months ago by Biostar ♦♦ 20
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Comment: C: BWA-mem alignment is not giving desired output
... now that i am trying with .fa indexed file. Here is the next error, that needs to be resolved: [E::bwa_set_rg] the read group line is not started with @RG and here is my read group: @A00234:29:H5LTLDMXX:2:2488:31855:37059 1:N:0:GCTGGTTA I read all the "passing a variable to the read group ...
written 14 months ago by micro32uvas10
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Comment: C: BWA-mem alignment is not giving desired output
... It just includes the intermediate processes. ...
written 14 months ago by micro32uvas10
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Comment: C: BWA-mem alignment is not giving desired output
... I am indexing the genome again with bwa. However, the command that you indicated; isnt it the same as i mentioned above? bwa mem -t 24 -M ref.fna trimmed-files1/1P.fastq trimmed-files1/2P.fastq >sample.sam 2> mem-pe.log Kindly elaborate it a little. I cannot get the desired output with t ...
written 14 months ago by micro32uvas10 • updated 14 months ago by genomax62k
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BWA-mem alignment is not giving desired output
... Hello, I have clean and trimmed fastq files with an average of 150nt per read. I have tried BWA aln and also BWA mem for the alignment with my reference genome. The alignment take around 2 days to process but ends u with 92kb file each time. I have changed algorithm from aln to mem, tried default ...
bwa-mem alignment wgs written 14 months ago by micro32uvas10 • updated 14 months ago by mittu1602150

Latest awards to micro32uvas

Popular Question 10 weeks ago, created a question with more than 1,000 views. For Viral Encoded miRNA prediction and target identification
Popular Question 11 weeks ago, created a question with more than 1,000 views. For Bowtie2 alignment error
Popular Question 13 months ago, created a question with more than 1,000 views. For bowtie2-align died with signal 6 (ABRT) (core dumped)

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