User: micro32uvas

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micro32uvas10
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New User
Location:
Pakistan
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2 days, 19 hours ago
Joined:
3 years, 7 months ago
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m**********@gmail.com

Posts by micro32uvas

<prev • 56 results • page 1 of 6 • next >
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Comment: C: Addition of Y chromosome to the ref sequence
... What I dont get, is that the providers of the ref seq, (NCBI and Bovine database) have not given any newer version for the said purpose (The latest came by April 2018, and its done by PacBio). Now one of the pipelines, shared by the 1000 Bull genome Project, includes the Ref Seq (NCBI's Ref seq+ Y) ...
written 4 months ago by micro32uvas10
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Addition of Y chromosome to the ref sequence
... Hello, I have aligned my WGS samples to a the latest bovine reference(B.tau5.0), available at NCBI. It is just yesterday, that in a pipeline used by 1000bull genome project, they have used the same reference merging the Y chromosome in it (B.taur5.0Y). Now, I am not sure about it. Should I align ...
assembly alignment bwa written 4 months ago by micro32uvas10 • updated 4 months ago by Devon Ryan91k
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Beauti and Beast
... Hello, I am working on bayesian analysis. I run BEAUTI and BEAST for MCMC runs for BSP analysis. I need to set 32% nucleotide substitution per million years but i cannot figure it out well. Can anyone help me out in the result interpretation. Ainnie ...
tracer nucleotide substitution per million year written 5 months ago by micro32uvas10 • updated 4 months ago by Biostar ♦♦ 20
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Arlequin interpretation for demography and molecular indices
... Hi there, I used Arlequin for a Molecular indices, demographic expansions, comparing the pairwise distances and pairwise Fst between 5 groups. Now that i got the results, I cannot figure out how to interpret them as well as if my deduced results are right. Can anyone help me in reading the arlequi ...
pairwisefst demographic expansion arlequin written 6 months ago by micro32uvas10
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Comment: C: NovoPlasty is producing identical Assemblies
... Yeah and to combat this issue, I went with Sanger for the confirmation. Turned out there was read depth problem at some locations, in case of multiple assemblies. They didn't vary much but few of them made multiple. Ruled the problem out by Sanger. ...
written 9 months ago by micro32uvas10
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Mitochondrial genomic analysis by PCoA, PCA and Arlequin
... Hello everyone, I have been working on the mitochondrial genome but it is not my expert field. I have extracted complete mitochondria from my whole genome sequenced samples. So far, I have used MEGA, DNASP and other related software. But I want to use Arlequin for population analysis but cannot f ...
organelle genome pca pcoa arlequin written 9 months ago by micro32uvas10
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NovoPlasty is producing identical Assemblies
... Hello, I have been extracting the mitochondria from my whole genome sequence data. This one sample, produced 3 assemblies, 2 of them are identical and the third one is close to the validated sequence (I did sanger to compare) It is seed and extend based and I cannot understand why it produced exa ...
assembly novoplasty mitochondria written 11 months ago by micro32uvas10 • updated 9 months ago by Biostar ♦♦ 20
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(Closed) Diff gene expression for knock out gene on RNA Seq data
... Hi there, I have got this RNA seq data in .csv format. doe degs. I need to find the knock out gene effect on this data. Can anyone help in a simplified manner? Awaiting your response. Ainnie NC1_length NC1_expected_count NC1_FPKM NC1_Symbol NC2_length NC2_expected_count NC2_FPKM NC2_Symbol ...
R degs knockout gene rna-seq written 15 months ago by micro32uvas10 • updated 14 months ago by Biostar ♦♦ 20
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Comment: C: BWA-mem alignment is not giving desired output
... now that i am trying with .fa indexed file. Here is the next error, that needs to be resolved: [E::bwa_set_rg] the read group line is not started with @RG and here is my read group: @A00234:29:H5LTLDMXX:2:2488:31855:37059 1:N:0:GCTGGTTA I read all the "passing a variable to the read group ...
written 19 months ago by micro32uvas10
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Comment: C: BWA-mem alignment is not giving desired output
... It just includes the intermediate processes. ...
written 19 months ago by micro32uvas10

Latest awards to micro32uvas

Popular Question 4 months ago, created a question with more than 1,000 views. For Convertion to gff3 format
Popular Question 4 months ago, created a question with more than 1,000 views. For Convertion to gff3 format
Popular Question 7 months ago, created a question with more than 1,000 views. For Viral Encoded miRNA prediction and target identification
Popular Question 7 months ago, created a question with more than 1,000 views. For Bowtie2 alignment error
Popular Question 18 months ago, created a question with more than 1,000 views. For bowtie2-align died with signal 6 (ABRT) (core dumped)

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