User: manojkumarbioinfo

Reputation:
20
Status:
New User
Location:
India
Last seen:
2 months ago
Joined:
2 years, 11 months ago
Email:
m****************@gmail.com

Posts by manojkumarbioinfo

<prev • 28 results • page 1 of 3 • next >
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Comment: C: Extracting Bar code From Fastq file
... I'm posting my Header file of my fastq. @M00196:24:000000000-AL55E:1:1101:14638:1425 1:N:0:4 TCTACCTACGGGTAGTCCAGATCGGAAGAGCACACGTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATGATATTCATTATATGTGACACGTCAATAGAAGCAATCAGCCAAGTGTCTGTAGCATGCTACTTTTTCTGTACAACAGACTTAATTTACTAAGCTATCCTATATGT ...
written 10 weeks ago by manojkumarbioinfo20 • updated 10 weeks ago by genomax58k
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Extracting Bar code From Fastq file
... Hai, I have a fastq file generated by Hiseq machine. i wish to extract the bar code of the sequence from the fastq files. ...
assembly next-gen sequencing written 10 weeks ago by manojkumarbioinfo20
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Comment: C: Error while aligning the sequence using Novoalign
... Thanks for your reply i sorted the problem with the read wile downloading the file using fastq-dump i set the quality as 30 that's why i faced the problem. ...
written 10 weeks ago by manojkumarbioinfo20
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Comment: C: Calculate the Quality of Bases
... sure i will look into it. ...
written 14 months ago by manojkumarbioinfo20
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Comment: C: Calculate the Quality of Bases
... ya that what i'm doing. but i want to know if there is any easier way than this. say if i have a 1000 variants like that we cant able to check them manually. ...
written 14 months ago by manojkumarbioinfo20
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Comment: C: Calculate the Quality of Bases
... i have used 5 different variant callers and merged every out put them i compared with my known ones, from that i missed about 86 variants which is not detected by any of my variant callers. so i wish to find whats the reason for missing those region. i checked with the depth, of those region it seem ...
written 14 months ago by manojkumarbioinfo20
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Comment: C: Calculate the Quality of Bases
... i'm not writing my own variant caller. i'm bench marking the available tools. for that i'm using my own sequence where i know the exact variant. but available tools are missing certain variants so i wish to find out the reason for missing variants. i checked the depth of those region it looks good b ...
written 14 months ago by manojkumarbioinfo20
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Comment: C: Calculate the Quality of Bases
... i just want to filter the variants based on the quality of the base and their depth. ...
written 14 months ago by manojkumarbioinfo20
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Calculate the Quality of Bases
... Hi, I want to calculate the quality of base using a bed file. for example **i have aligned my raw reads and i want to calculate the quality of base found in chromosome 1 at particular position**. is there any tools for calculating the quality of user desired bases. if yes kindly suggest me the be ...
next-gen alignment sequencing written 14 months ago by manojkumarbioinfo20 • updated 14 months ago by prasundutta87260
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Error while aligning the sequence using Novoalign
... Hi, I'm getting a error name base quality out of range . while aligning my reads using Novoalign. **Command used for aligning** novoalign -d Reference/Novoalign/hg37.nix -f data/SRR504483_1.fastq data/SRR504483_2.fastq -i 200,50 -o SAM **Error message** Error: Base quality out of range for file ...
next-gen ngs alignment written 18 months ago by manojkumarbioinfo20 • updated 10 weeks ago by Sparks70

Latest awards to manojkumarbioinfo

Popular Question 9 weeks ago, created a question with more than 1,000 views. For VCF - compare
Popular Question 14 months ago, created a question with more than 1,000 views. For VCF - compare
Popular Question 14 months ago, created a question with more than 1,000 views. For Comparing VCF with dbsnp
Popular Question 14 months ago, created a question with more than 1,000 views. For Incompatible contigs No overlapping contigs found.
Popular Question 17 months ago, created a question with more than 1,000 views. For Incompatible contigs No overlapping contigs found.
Popular Question 17 months ago, created a question with more than 1,000 views. For Difference Between GATK, Freebayes and SAMtools mpileup
Scholar 2.7 years ago, created an answer that has been accepted. For A: Difference Between GATK, Freebayes and SAMtools mpileup

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