User: manojkumarbioinfo

Reputation:
60
Status:
New User
Location:
India
Last seen:
2 weeks ago
Joined:
4 years, 5 months ago
Email:
m****************@gmail.com

Posts by manojkumarbioinfo

<prev • 32 results • page 1 of 4 • next >
3
votes
2
answers
125
views
2
answers
Answer: A: How to remove sequence reads contains more than 1 X from multifasta file?
... > awk -v RS='>' '!/XX/{printf $0RT}' test.fasta **Output** Prot1 ANSTVKKKKLLLYYYSSSEERXFGHYFGHYFGHFYVHFGFYVHCEDYHF Prot3 ANSTVKKKKLLLYYYSSSEERFGHYFGHYFGHFYVHFGFYVHCEDYHF ...
written 18 days ago by manojkumarbioinfo60
0
votes
0
answers
298
views
0
answers
Comment: C: Error, fewer reads in file specified with -2 than in file specified with -1
... I used BBmap repair.sh in1=r1.fq.gz in2=r2.fq.gz out1=fixed1.fq.gz out2=fixed2.fq.gz outsingle=singletons.fq.gz to fix this issue ...
written 5 months ago by manojkumarbioinfo60
0
votes
0
answers
1.8k
views
0
answers
Comment: C: Error with Bowtie 2
... It worked fine thank you. the reads were not matching that's the problem ...
written 5 months ago by manojkumarbioinfo60
2
votes
0
answers
298
views
0
answers
Error, fewer reads in file specified with -2 than in file specified with -1
... Hai, I'm using bowtie2 as my aliner to align my sequence. I'm getting an error Fewer reads in the file specified with -2 than in file specified with -1. i used the same file to align with BWA-mem it does not show any error. but botie2 is still running for more than 1week with the same file size. t ...
wgs ngs sequencing bowtie2 written 6 months ago by manojkumarbioinfo60 • updated 6 months ago by h.mon29k
0
votes
0
answers
1.2k
views
0
answers
Comment: C: Extracting Bar code From Fastq file
... I'm posting my Header file of my fastq. @M00196:24:000000000-AL55E:1:1101:14638:1425 1:N:0:4 TCTACCTACGGGTAGTCCAGATCGGAAGAGCACACGTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATGATATTCATTATATGTGACACGTCAATAGAAGCAATCAGCCAAGTGTCTGTAGCATGCTACTTTTTCTGTACAACAGACTTAATTTACTAAGCTATCCTATATGT ...
written 21 months ago by manojkumarbioinfo60 • updated 21 months ago by genomax83k
1
vote
0
answers
1.2k
views
0
answers
Extracting Bar code From Fastq file
... Hai, I have a fastq file generated by Hiseq machine. i wish to extract the bar code of the sequence from the fastq files. ...
assembly next-gen sequencing written 21 months ago by manojkumarbioinfo60
0
votes
1
answer
919
views
1
answers
Comment: C: Error while aligning the sequence using Novoalign
... Thanks for your reply i sorted the problem with the read wile downloading the file using fastq-dump i set the quality as 30 that's why i faced the problem. ...
written 21 months ago by manojkumarbioinfo60
0
votes
1
answer
1.6k
views
1
answers
Comment: C: Calculate the Quality of Bases
... sure i will look into it. ...
written 2.7 years ago by manojkumarbioinfo60
0
votes
1
answer
1.6k
views
1
answers
Comment: C: Calculate the Quality of Bases
... ya that what i'm doing. but i want to know if there is any easier way than this. say if i have a 1000 variants like that we cant able to check them manually. ...
written 2.7 years ago by manojkumarbioinfo60
0
votes
1
answer
1.6k
views
1
answers
Comment: C: Calculate the Quality of Bases
... i have used 5 different variant callers and merged every out put them i compared with my known ones, from that i missed about 86 variants which is not detected by any of my variant callers. so i wish to find whats the reason for missing those region. i checked with the depth, of those region it seem ...
written 2.7 years ago by manojkumarbioinfo60

Latest awards to manojkumarbioinfo

Scholar 18 days ago, created an answer that has been accepted. For A: Difference Between GATK, Freebayes and SAMtools mpileup
Teacher 18 days ago, created an answer with at least 3 up-votes. For A: How to remove sequence reads contains more than 1 X from multifasta file?
Popular Question 4 months ago, created a question with more than 1,000 views. For Regarding Depth coverage
Popular Question 4 months ago, created a question with more than 1,000 views. For VCF - compare
Popular Question 20 months ago, created a question with more than 1,000 views. For VCF - compare
Popular Question 20 months ago, created a question with more than 1,000 views. For Regarding Depth coverage
Popular Question 20 months ago, created a question with more than 1,000 views. For Comparing VCF with dbsnp
Popular Question 20 months ago, created a question with more than 1,000 views. For VCF - compare
Popular Question 2.7 years ago, created a question with more than 1,000 views. For VCF - compare
Popular Question 2.7 years ago, created a question with more than 1,000 views. For Comparing VCF with dbsnp
Popular Question 2.7 years ago, created a question with more than 1,000 views. For Incompatible contigs No overlapping contigs found.
Popular Question 3.0 years ago, created a question with more than 1,000 views. For Incompatible contigs No overlapping contigs found.
Popular Question 3.0 years ago, created a question with more than 1,000 views. For Difference Between GATK, Freebayes and SAMtools mpileup
Scholar 4.2 years ago, created an answer that has been accepted. For A: Difference Between GATK, Freebayes and SAMtools mpileup

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1764 users visited in the last hour