User: jmirobla

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jmirobla0
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Posts by jmirobla

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Comment: C: conactenate/merge fastq files
... Many thanks! And this would also work with gzip compressed files just adding a gunzip/gzip command right? ...
written 3 months ago by jmirobla0
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Comment: C: conactenate/merge fastq files
... file1 is the UMIs fastq file separated from the file2 that is the actual read, I need to put them together again ...
written 3 months ago by jmirobla0
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conactenate/merge fastq files
... I want would like to merge or concatenate reads from 2 fastq files in order to have the following output file 1 @J00148:193:HFG7MBBXY:2:1101:2372:1279 2:N:0:GAAGGAAG+GACGTCAT AANCTTCCC + -<#FFFFFA file 2 @J00148:193:HFG7MBBXY:2:1101:2372:1279 1:N:0:GAAGGAAG+GACGTCAT GT ...
next-gen alignment rna-seq written 3 months ago by jmirobla0 • updated 3 months ago by ATpoint35k
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Help with 2nd read data analysis
... Analysing 2nd read from a V3 run on a HiSeq and I've goot the below image about quality: ![enter image description here][1] Could anyone let me know if it is ok to trim the last 5-10 bases? The data woul still be usefull? Many thanks [1]: http://s9.postimg.org/419yhqedp/2nd_read_quality.png ...
sequence sequencing written 4.2 years ago by jmirobla0 • updated 4.2 years ago by Devon Ryan95k

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