User: clizama

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clizama0
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Posts by clizama

<prev • 17 results • page 1 of 2 • next >
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ATAC-SEQ primers order
... HI, I'm starting a experiment with some cells from mice embryos. I want to be sure, my primers are correct before to do all the experiment. I'm a little confused how to orders the primers for ATAC-seq: For each sample, I should be order: Sample1: Ad1_noMX: (Primer without index) Ad2.1_index: ...
chip-seq written 7 weeks ago by clizama0
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Comment: C: ENSDAR genes to MGI symbols
... Thanks, I will try Ensembl's BioMart. ...
written 12 weeks ago by clizama0
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ENSDAR genes to MGI symbols
... HI, Has any good tool to convert the ENSDAR gene names in MGI symbols? Thanks ...
gene written 12 weeks ago by clizama0 • updated 12 weeks ago by Jean-Karim Heriche15k
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Comment: C: ebi.uk RNAseq data
... It's possible these are replicates for the same sample, I checked the bioproject in NIH the experiment has 15 SRA files, but the ebi website has 28. ...
written 3 months ago by clizama0
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Comment: C: ebi.uk RNAseq data
... Hi, Just checking the FTP column, If you check the code SRR5314617 and SRR5314618 both correspond to same sample ( RNA-seq liver macrophage 1m old liver1mWT3) and both has a single set of paired-end files. Then, I'm little confused if they split the files in two set of paired end, and I have to mer ...
written 3 months ago by clizama0
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ebi.uk RNAseq data
... Hey, I was downloading some pair end data files from ebi.ac.uk. I have the following problem in general they have just two files per sample (for pair end). This RNAseq has 4 files per sample I think they split the files in 4, two for each files. https://www.ebi.ac.uk/ena/data/view/PRJNA378234 But ...
rna-seq written 3 months ago by clizama0 • updated 3 months ago by Devon Ryan79k
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Comment: A: Galaxy for RNASEQ CHIPSeq analysis
... thank you, I will do it. ...
written 5 months ago by clizama0
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Galaxy for RNASEQ CHIPSeq analysis
... Hi, I used to use galaxy in amazon web services (from UCDAVIS). Looks like the public AMI is not running property anymore. I was wondering if has any other public AMI in amazon web services to run a galaxy instances or in other cloud. I was trying to use cloudman to run a galaxy instances in a ...
galaxy written 5 months ago by clizama0 • updated 16 days ago by leninchrist980
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Fr-unstranded vs Fr-first strand
... Just curiosity: I know some Library preps kits are Strand specific and others no. In the case of strand specific, for example FR-First strand What happen if I do a analysis using FR-unstraded and FR-first strand for the same data using top-hat. I should have different numbers of count with HT- ...
rna-seq written 8 months ago by clizama0 • updated 8 months ago by Kevin Blighe19k
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Comment: C: tophat error help
... Yeah I'm working in try to change the number of threads, my problem is Im runing tophat in galaxy and I couldnt find the option to change the number of threads from 8 to 1. ...
written 2.2 years ago by clizama0

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Popular Question 8 months ago, created a question with more than 1,000 views. For tophat error help

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