User: lay_0

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lay_020
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Posts by lay_0

<prev • 18 results • page 1 of 2 • next >
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Answer: A: Branch Lengths in RAxML
... I believe is number of changes per site, this is probably the same question: https://www.biostars.org/p/52359/ ...
written 9 days ago by lay_020
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Comment: C: "three genes" exemple in genoplotR
... I do have the same question and I did, in the past worked with R. I would really like to see how the "three_genes" dataset looks like, I tried several things but for some reason is not an object.... did you find your answer?... ...
written 4 months ago by lay_020
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Comment: C: something similar to FancyGene
... I did contact her, no answer yet. Thanks! ...
written 4 months ago by lay_020
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Comment: C: something similar to FancyGene
... Thanks, indeed it seems like is just maintenance problem. I did contacted the author before posting here but didn't get any answer. ...
written 4 months ago by lay_020
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something similar to FancyGene
... Hi, I used FancyGene in the past to graphically display genes and features that are close together in a sequence, and compare that genomic region between species on a scale. Well, FancyGene seems to be not working anymore, I think the person in charge has moved to a different University and the webs ...
gene structure gene clusters visualization tool written 4 months ago by lay_020 • updated 4 months ago by Michael Dondrup41k
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extract genomic sequence based on transcripts
... Hello, Can someone tell me how I can extract a full genomic sequence (intron, exons, full gene) if I have a set of transcripts and the genome in FASTA format both?. At the least the genomic coordinates for each of the genes only would work. thanks ...
genome fasta coordinates extract transcripts written 9 months ago by lay_020 • updated 9 months ago by Ram11k
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Comment: C: TMM - Normalized FPKM values for RNASeq data ?
... I have the same question as gangireddy, but how can you enter FPKMs in edgeR?, just as you would do with raw counts?, never mind the non-integers? ...
written 9 months ago by lay_020
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Comment: A: mapping initial libraries to Trinity assembly
... yes, trimmomatic output was used for the assembly. thanks, that is what I suspected....i need to repeat the RSEM. I was hoping some one would say it didn't matter. If my reads were already trimmed for adapters, and they are of really good quality before trimming... do you think is still a problem ...
written 10 months ago by lay_020
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Comment: A: mapping initial libraries to Trinity assembly
... I run trimmomatic separately, before starting Trinity, I may have missed some output there :( ...
written 10 months ago by lay_020
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mapping initial libraries to Trinity assembly
... I am worrying about something I came up while writing down my methods: I assembled a transcriptome for a non-model species using the trinity pipeline. Adapters were removed at the sequencing center so I didn't have to worry about that. I checked at the quality of the libraries with fastqc and decid ...
trinity rna-seq rsem written 10 months ago by lay_020 • updated 9 months ago by Biostar ♦♦ 10

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