User: Muhammad Haseeb
Muhammad Haseeb • 50
- Reputation:
- 50
- Status:
- New User
- Location:
- Ajou University, Suwon, South Korea
- Website:
- http://openwetware.org...
- Last seen:
- 4 months, 3 weeks ago
- Joined:
- 1 year, 5 months ago
- Email:
- h*********@hotmail.com
I'm Ph.D. Research Scholar in Innate Immunity and Cell Signaling Lab, with a strong interest in Systems Biology and Bio-informatics. I mainly work on Biological Signalling Pathways, Mathematical Modelling and Simulations, Proteomics data and Computer Aided Drug Designing (CADD). I have the most experience with MATLAB (Simulink), Data Structure but starting to get rather efficient in R.
Posts by Muhammad Haseeb
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... Dear b.nota,
Thank you very much. The data format are as follow
{Reporter Identifier} {RATIO} {G_PROCESSED} {R_PROCESSED}
1 [-0.148533] [42903.2] [30475.9]
........................
...
written 4 months ago by
Muhammad Haseeb • 50
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... Hi,
I have 33 ligands in total, which were analyzed through SAM. Reported in an article entitled "**Analysis of the major patterns of B cell gene expression changes in response to short-term stimulation with 33 single ligands**". I selected 10 ligands from above data and wants additional analysis b ...
written 4 months ago by
Muhammad Haseeb • 50
• updated
3 months ago by
Biostar ♦♦ 20
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... Dear Wounter,
Thank you for the answer, Can you clarify me, I didn't understand what do you mean by all time points against the first? You mean all time point that is 40 columns of fold change for each time, compare with control?
For example: (30min_fold change of **normal** Vs 30min_fold change of ...
written 5 months ago by
Muhammad Haseeb • 50
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... Hi,
I hope you all are doing good. My question is that I have log2 gene expression data of four different time span (30min, 1hr, 2hr and 4hr). I want to calculate the Fold change (FC) and P-value for it, Can I calculate FC individually for each time. For example (30min_FC,1hr_FC, 2hr_FC and 4hr_FC) ...
written 5 months ago by
Muhammad Haseeb • 50
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... Dear H.Mon,
Thank you very much for a nice answer. Actually, my concern is that **can I use the differential expressed gene (DEG) supplementary file of this data for further analysis**? If yes, then the supplementary file is missing the gene symbols and they mentioned only the feature number and gen ...
written 5 months ago by
Muhammad Haseeb • 50
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... I am working on microarray data analysis of B cell (GSE2050). They didn't mention the probe set id,...etc (unique identifier) for gene annotation/gene symbol, they analyze the data through significance analysis of Microarray (SAM) and reported DEG (2937) in the supplementary file as follows [feature ...
written 5 months ago by
Muhammad Haseeb • 50
• updated
5 months ago by
h.mon ♦ 8.7k
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... Dear Nathan,
Thank you very much for such a nice share. I will follow your suggestions and will contact if needed.
Thank you,
Regards, ...
written 8 months ago by
Muhammad Haseeb • 50
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317
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... Thank you very much for providing the link.
Regards, ...
written 8 months ago by
Muhammad Haseeb • 50
1
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... Hey Everyone,
I hope you all are doing good. I am interested in analyzing microarray data of tumor gene expression. I want to identify the differentially expressed gene among **wildtype** VS **Knockout**. And to check their role, I used the **AltAnalyze** tool for normalization, heatmap, clustering ...
written 8 months ago by
Muhammad Haseeb • 50
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... Dear Researcher,
I am analyzing the microarray data of mouse_402 by using **Affymetrix console Expression** and normalized through RMA.We have total 8 chip, **4 Wild type** (WT day 4 (r1),WT day 4 (r2),WT day 6 (r1), WT day 6 (r2)) and **4 Knockout** similarly (KO day 4 (r1)...........). After gene ...
written 8 months ago by
Muhammad Haseeb • 50
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