User: alim.hcu

gravatar for alim.hcu
alim.hcu10
Reputation:
10
Status:
New User
Location:
Last seen:
1 month, 3 weeks ago
Joined:
3 years, 3 months ago
Email:
a*******@gmail.com

Posts by alim.hcu

<prev • 42 results • page 1 of 5 • next >
0
votes
1
answer
111
views
1
answers
Comment: C: RNA Seq analysis of rice samples
... We did RNA-Seq for DEG analysis. ...
written 8 weeks ago by alim.hcu10
1
vote
1
answer
111
views
1
answer
RNA Seq analysis of rice samples
... Dear all I have RNA-seq data from rice crop which is model and well annotated crop. When i mapped the reads, i found that most of reads(70-80%) are multi mapped. I also observed that most of the multi mapped reads are coming from the genic region. What may be the reason for such type of multi mappe ...
alignment rna-seq sequencing written 8 weeks ago by alim.hcu10 • updated 8 weeks ago by guillaume.rbt620
0
votes
0
answers
252
views
0
answers
Comment: A: RNA Seq analysis of rice samples
... Thanks for your kind reply.... As you suggested that i should proceed with HIsat2. Here i got following output from hisat2. I think the problem is with multimapped reads. Due to 49% multimapped reads, only 35% reads aligned uniquely, so less number of reads for quantification (as htseq-count quanti ...
written 3 months ago by alim.hcu10
0
votes
0
answers
252
views
0
answers
Comment: C: RNA Seq analysis of rice samples
... RNA was isolated from 10 day old seedling plant. We have average 50 million reads per sample. ...
written 4 months ago by alim.hcu10
0
votes
0
answers
252
views
0
answers
Comment: C: RNA Seq analysis of rice samples
... Sorry..it was typo error. .less than one.. ...
written 4 months ago by alim.hcu10
4
votes
0
answers
252
views
0
answers
RNA Seq analysis of rice samples
... Dear I am analysing a RNA sequencing data of rice samples. Sequenced were mapped using tophat and most of sample had more than 90% mapping rate (although there were 50% multimapped reads). When I used cufflink for quantification of genes, i found more than 90% genes showed fpkm value less than zero ...
next-gen rna-seq sequencing written 4 months ago by alim.hcu10
0
votes
0
answers
287
views
0
answers
Comment: C: binomial probability distribution, the one-tailed P value
... Formula link ...
written 15 months ago by alim.hcu10 • updated 15 months ago by genomax70k
0
votes
0
answers
287
views
0
answers
Comment: C: binomial probability distribution, the one-tailed P value
... https://ibb.co/cvinzS ...
written 15 months ago by alim.hcu10
0
votes
0
answers
287
views
0
answers
Page: (Closed) binomial probability distribution, the one-tailed P value
... ![enter image description here][1] [1]: https://image.ibb.co/eyABR7/Screenshot_from_2018_05_06_17_20_56.png I want to one-tailed P value by binomial probability distribution. I have two value of nCG and mCG. Lets assume nCG=10 and mCG=5. Any script in R? ...
page R written 15 months ago by alim.hcu10 • updated 15 months ago by RamRS23k
0
votes
1
answer
651
views
1
answers
Comment: A: LTR insertion time
... One recently published paper estimate the insertion time by "DNA divergence between the sequence was estimated with the baseml program from PAML ver. 4.8 (Yang 2007) using the Kimura-2-parameter base substitution model" Since i am new in bioinfo specially in evolution. So i am not able to understand ...
written 16 months ago by alim.hcu10

Latest awards to alim.hcu

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1687 users visited in the last hour