User: candida.vaz
candida.vaz • 0
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Posts by candida.vaz
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... I have also used STAR aligner for mapping. Thanks for the information, will look up the publication you suggested. ...
written 20 months ago by
candida.vaz • 0
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... Hello everyone,
I have Illumina paired end (2*150bp) reads for 30 samples that I mapped to the reference genome using Tophat2. The sequencing providers gave me the following information:
- The fragments used for sequencing range from 200 to 400bp
- The average size is around 303 to 330bp. This inc ...
written 20 months ago by
candida.vaz • 0
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Hello Devon,
Thanks for your answer! I was wondering what is feature count? I am using Htseq-count.
http://www-huber.embl.de/HTSeq/doc/count.html
While running STAR **--outSAMtype BAM Unsorted SortedByCoordinate** we get two BAM files
STAR manual recommends to use the output **Unsorted bam** file ...
written 2.2 years ago by
candida.vaz • 0
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Dear All,
I am using STAR aligner for mapping my paired end reads using the following parameters:
/cluster/apps/x86_64/packages/STAR_2.5/bin/STAR --runThreadN 4 --genomeDir /home/candidav/STAR-Gallus-gallus-nv5/Gallus-gallus-genome-dir-nv5 --readFilesIn /home/candidav/S1/RCI001-forward_paired.fas ...
written 2.2 years ago by
candida.vaz • 0
• updated
2.2 years ago by
Devon Ryan ♦ 91k
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... Hello everyone,
Is anyone familiar with the **"keepBothReads"** in **Trimmomatic** :
**keepBothReads:**
After read-through has been detected by palindrome mode, and the adapter sequence removed, the reverse read contains the same sequence information as the forward read, albeit in reverse complem ...
written 2.3 years ago by
candida.vaz • 0
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2.3 years ago by
Brian Bushnell ♦ 16k
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... https://support.illumina.com/bulletins/2016/12/what-sequences-do-i-use-for-adapter-trimming.html ...
written 2.3 years ago by
candida.vaz • 0
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... Oh I see!
But did you check your R1 and R2 files for the presence of the adapters mentioned by Illumina.
My only issue is the sequence mentioned in the trimmomatic adapter file as 2 is actually for R1 and vice versa.
...
written 2.3 years ago by
candida.vaz • 0
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... Hi Macspider,
Could you please share the adapter file you used for removing adapters from Truseq PE data using Trimmomatic.
Was your file similar to mine or did you use the "TruSeq3-PE-2.fa".
Though the sequences and their reverse complements are the same, but if you notice, they are for opposite ...
written 2.3 years ago by
candida.vaz • 0
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So did you have the "TACACTCTTTCCCTACACGACGCTCTTCCGATCT" adapter seq in your R1 file and
"GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT" seq in your R2 file?
Could you check taking the top 200~ sequences of both your files ...
written 2.3 years ago by
candida.vaz • 0
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... Yes it is also mentioned in the manual that one needs to mention the reverse complement.
My doubt is regarding the universal usage of the Trimmomatic provided adapter file "TruSeq3-PE-2.fa".
Should this file be used always? Or was I right in making my own file according to the adapters in my file.
...
written 2.3 years ago by
candida.vaz • 0
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19 months ago,
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For High number of no feature while using htseq-count on Tophat2 aligned as well as STAR aligned paired-end data
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For Trimmomatic adapter file TruSeq3-PE-2.fa
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