User: nsl24

gravatar for nsl24
nsl240
Reputation:
0
Status:
New User
Last seen:
4 months ago
Joined:
1 year, 12 months ago
Email:
n****@scarletmail.rutgers.edu

Profile information, website and location are not shown for new users.

This helps us discourage the inappropriate use of our site.

Posts by nsl24

<prev • 6 results • page 1 of 1 • next >
0
votes
0
answers
139
views
0
answers
goseq for non-native species infinite recursion
... I'm trying to used the results of a differential expression analysis to look for enriched genes using goseq but I'm having a beast of a time even getting a trial for my non-native species working. I have: - downloaded gene lengths as a numeric vector taken from biomart (Length) - A gene.vecto ...
software error goseq rna-seq written 4 months ago by nsl240
0
votes
0
answers
229
views
0
answers
Can I connect RNAseq data to comparative genomics questions?
... It was recently pointed out to me that a RNA-seq dataset I've been working on could have interesting implications for evaluating the evolution of traits in a species related to my species of interest. Both species have well described reference genomes and I'm considering incorporating some comparati ...
rna-seq written 5 months ago by nsl240
0
votes
1
answer
247
views
1
answers
Comment: C: Dropping treatments with low alignment from differential expression analyses?
... Thanks for the warning Kenneth. The "bad" treatment was a exploratory addition to the other two treatments (which are really my focus) and isn't really necessary, I'm just trying to see if it COULD work in its current form. Culturing the lines for the third treatment was difficult, getting rid of t ...
written 5 months ago by nsl240
0
votes
1
answer
247
views
1
answers
Comment: C: Dropping treatments with low alignment from differential expression analyses?
... Thanks for the reply, and sorry for my leaving a gap in my description. My good treatments range between 10M and 12M reads aligning, while my bad treatment ranges between 1.2M and 6M aligning. Does that change your perspective? Do you have any suggestions for how to become better at making these s ...
written 5 months ago by nsl240
3
votes
1
answer
247
views
1
answer
Dropping treatments with low alignment from differential expression analyses?
... I have the downstream plan of performing differential expression analyses (using rDiff and Deseq2) on a data set containing three treatments which each have three replicates. Two of the treatments aligned very well (>87% aligned unambiguously in STAR). One treatment, however, has a contaminant ( ...
alignment rna-seq written 5 months ago by nsl240 • updated 5 months ago by WouterDeCoster29k
0
votes
0
answers
574
views
0
answers
Pooling samples across timepoints
... Sorry if this is anyone's peeve question, but my search has largely turned up mixed responses. I have a single-celled eukaryotic system with a plastic phenotype. I want to induce the phenotype to two different degrees and then have a non-induced control and do a DE. So that would be: High treatmen ...
rna-seq written 24 months ago by nsl240

Latest awards to nsl24

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 590 users visited in the last hour