User: Rduncan

gravatar for Rduncan
Rduncan60
Reputation:
60
Status:
Trusted
Location:
Miami, FL
Website:
http://www.bio.miami.e...
Last seen:
1 year, 3 months ago
Joined:
5 years, 11 months ago
Email:
r**********@gmail.com

I am a PhD candidate at the University of Miami. I use comparative and functional genomics to understand how insects interact with their bacterial symbionts and how insect genomes have been shaped by symbiosis.

Posts by Rduncan

<prev • 7 results • page 1 of 1 • next >
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Comment: C: Using RNAseq to measure gene expression in a tissue when the gene is NOT differe
... That is a very interesting idea.  Thanks for sharing!   ...
written 22 months ago by Rduncan60
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Comment: C: Using RNAseq to measure gene expression in a tissue when the gene is NOT differe
... Thanks Devon.  Wouldn't relative quantification with qPCR be more appropriate though? If I want to know if the gene is highly expressed in bacteriocyte, it seems like I would need to compare it to something like a housekeeping gene. Then I couldn't quantitatively compare across species, but it is mo ...
written 22 months ago by Rduncan60
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Using RNAseq to measure gene expression in a tissue when the gene is NOT differentially expressed
... There is a lot of interest in tissue-specific and differentially expressed genes and a lot of people are using RNAseq to determine if genes are upregulated in one tissue vs. another.  However, I am interested in a particular gene that, based on qPCR, is highly expressed in a particular aphid tissue ...
gene expression comparison within tissue rna-seq written 22 months ago by Rduncan60 • updated 6 months ago by Biostar ♦♦ 20
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Comment: C: What Is The N50 Contig Number (Not N50 Length)?
... OK, I see. Thanks for your answer! ...
written 5.5 years ago by Rduncan60
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Comment: C: How To Remove The Same Sequences In The Fasta Files?
... Does this script only collapse sequences that are completely overlapping or will it collapse sequences where there is only partial overlap but the overlapping region is redundant? ...
written 5.5 years ago by Rduncan60
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Comment: C: What Is The N50 Contig Number (Not N50 Length)?
... It seems like this is a slightly different, and perhaps more precise, way of saying what other people have answered: that it is the number of contigs >= N50 length. ...
written 5.7 years ago by Rduncan60
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What Is The N50 Contig Number (Not N50 Length)?
... I understand the meaning of N50 contig length, but lately I've been coming across assembly statistics that report the N50 contig number. Can someone explain what is meant by N50 contig number and how it relates to assembly quality? ...
assembly written 5.7 years ago by Rduncan60 • updated 5.7 years ago by Fabian Bull1.3k

Latest awards to Rduncan

Appreciated 4.6 years ago, created a post with more than 5 votes. For What Is The N50 Contig Number (Not N50 Length)?
Good Question 4.6 years ago, asked a question that was upvoted at least 5 times. For What Is The N50 Contig Number (Not N50 Length)?
Popular Question 4.6 years ago, created a question with more than 1,000 views. For What Is The N50 Contig Number (Not N50 Length)?
Student 4.6 years ago, asked a question with at least 3 up-votes. For What Is The N50 Contig Number (Not N50 Length)?
Autobiographer 4.6 years ago, has more than 80 characters in the information field of the user's profile.

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