User: Picoskia

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Picoskia0
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Posts by Picoskia

<prev • 11 results • page 1 of 2 • next >
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Comment: C: Calculate Tag density
... i just want the tag/read numbers ...
written 9 days ago by Picoskia0
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Comment: C: Calculate Tag density
... I would like to plot the tag density for a certain group of genes. For this i need the tag count as well as the normalised read density as you said. I have looked to different tools but haven't found where to get the tag count information ...
written 9 days ago by Picoskia0
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Calculate Tag density
... Hi everyone, I was wondering how to calculate the density over a specific list of genes. Which file should i use to start this analysis and how do i count the tags? I have MACS2 output file, is there a column in those file where tag count is specified for each of the peaks? Thank you for your kind ...
tag genes chip-seq written 9 days ago by Picoskia0 • updated 9 days ago by WouterDeCoster34k
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Comment: C: Nomalize chip-seq data
... Thank you for your answers, so if i understand the normalization step has to be done after the alignment when calling peaks with MACS. ...
written 14 months ago by Picoskia0
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Comment: C: Nomalize chip-seq data
... The three ChIPseq data are the same factor : one WT and two with mutations ...
written 14 months ago by Picoskia0
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Nomalize chip-seq data
... Hi everyone, I have to analyze 3 chip-seq datasets. I'm fine with the analysis procedure itself but have a question about the normalization. At which step(s) should i normalize my datas? - Before the alignment by sequencing depth? - At the peak calling step with MACS? - At the conversion step from ...
chip-seq written 14 months ago by Picoskia0 • updated 14 months ago by jared.andrews071.5k
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Comment: C: Only phiX reads in my data
... i have give them the sequencing primers maybe my library went wrong ...
written 2.0 years ago by Picoskia0
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Comment: C: Only phiX reads in my data
... unfortunately no...... ...
written 2.0 years ago by Picoskia0
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Comment: C: Only phiX reads in my data
... thank you for your answer, i should try to make a new library.... ...
written 2.0 years ago by Picoskia0
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Comment: C: Only phiX reads in my data
... Thanks a lot, yes i have prepared a custom library with specific primers. Do you have any clue about why my reads are not mapping to my reference genome in this case? ...
written 2.0 years ago by Picoskia0

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